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US 5,654,426 AGranted
Ecteinascidins
OwnerKenneth L. Rinehart, Ryuichi Sakaiinventors
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Abstract
Ecteinascidins (Et's), isolated from the Caribbean tunicate Ecteinascidia turbinata, protect mice in vivo against P388 lymphoma, B16 melanoma, M5076 ovarian sarcoma, Lewis lung carcinoma, and the LX-1 human lung and MX-1 human mammary carcinoma zenografts.
Claims2
A process for the preparation of ecteinascidin 756 consisting of the steps of:
- (a) extracting homogenized E. turbinata with methanol,
- (b) treating the methanol extract with KCN,
- (c) adding an aqueous NaCl solution to the methanol solution,
- (d) partitioning the water-methanol mixture with toluene,
- (e) extracting the aqueous phase with dichloromethane,
- (f) evaporating the dichloromethane solvent, dissolving the residue in methanol and extracting the methanol with hexane;
- and
- (g) separating the compound having the following formula from the methanol solution by chromatographic means ##STR2##
5 more elements
A process for the preparation of ecteinascidin 770 consisting of the steps of:
- (a) extracting homogenized E turbinata with methanol,
- (b) treating the methanol extract with DCN,
- (c) adding an aqueous NaCl solution to the methanol solution,
- (d) partitioning the water-methanol mixture with toluene,
- (e) extracting the aqueous phase with dichloromethane,
- (f) evaporating the dichloromethane solvent, dissolving the residue in methanol and extracting the methanol with hexane;
- and
- (g) separating the compound having the following formula from the methanol solution by chromatographic means ##STR3##
5 more elements
Dependencies are inferred from the claim text.