Device and process for isolating nucleic acids from cell suspension
AssigneeOiagen GmbH
InventorsKarsten Henco, Metin Colpan, Petra Feuser
Life of this patent
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Abstract
A device and a process for isolating nucleic acids by lysing intact cells and removing nucleic acids emerging from the lysed cells by the following steps: a) the cells are immobilized in a porous matrix, with the size of matrix voids being in the range of the type of cell to be lysed; b) the cells are lysed; c) the nucleic acids are fixated on the matrix surface, and subsequently d) are eluted.
Claims12
A process for isolating nucleic acids by lysing blood cells, substantially all of which are intact, and removing the nucleic acids emerging from the lysed cells, comprising the steps of:
- a) immobilizing the cells in a porous matrix having ion exchange properties and having voids each having a size sufficient to capture one of the cells;
- b) lysing the cells;
- c) fixing the emerging nucleic acids on the matrix surface by ion exchange, and subsequently;
- d) eluting the nucleic acids.
In a process for isolating nucleic acids by lysing blood cells and removing the nucleic acids emerging from the lysed cells, the improvement comprising the steps of:
- a) before lysing, immobilizing the blood cells in a porous matrix having ion exchange properties and having voids each having a size sufficient to capture one of the cells;
- b) lysing the captured cells;
- c) fixing the emerging nucleic acids on the matrix surface by ion exchange, and subsequently;
- d) eluting the nucleic acids.
Dependencies are inferred from the claim text.