Specific immune system modulation
AssigneeYale University
InventorsRichard L. Edelson, Francis P. Gasparro, Robert E. Tigelaar
Life of this patent
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Abstract
Methods and pharmaceutical compositions for modifying the immune response of a mammal to a specific antigen are provided. The methods include treating an antigen presenting cell to enhance expression of a major histocompatibility complex molecule and reacting the treated antigen presenting cell with the antigen extracorporeally to form an antigen-associated antigen presenting cell.
Claims12
A method for forming a peptide antigen-associated leukocyte, comprising the steps of:
- (a) irradiating a preparation containing a mixture of leukocytes in the presence of a psoralen at a temperature between about 20.degree. C. and about 28.degree. C., wherein the mixture of leukocytes is withdrawn from a mammal;
- and followed by
- (b) extracorporeally reacting the irradiated mixture of leukocytes with a mixture of autologous peptide antigens associated with autoimmune disease, allergic reactions, or hypersensitivity disease to form a plurality of the peptide antigen-associated leukocytes, wherein the autologous peptide antigens are obtained from said mammal.
A method for making a pharmaceutical preparation for administration to a mammal, comprising the steps of:
- (a) irradiating a preparation containing a mixture of leukocytes in the presence of a psoralen at a temperature between about 20.degree. C. and about 28.degree. C., wherein the mixture of leukocytes is withdrawn from said mammal;
- followed by
- (b) extracorporeally reacting the irradiated mixture of leukocytes with a mixture of autologous peptide antigens associated with autoimmune disease, allergic reactions, or hypersensitivity disease to form a plurality of the peptide antigen-associated leukocytes, wherein the autologous peptide antigens are obtained from said mammal;
- and
- (c) placing the peptide antigen-associated leukocytes in a pharmaceutically acceptable carrier.
A method for specifically down-regulating the immune system T-cell response of a mammal to an autologous peptide antigen, comprising the steps of:
- (a) irradiating a preparation containing a mixture of leukocytes in the presence of a psoralen at a temperature between about 20.degree. C. and about 28.degree. C. wherein the mixture of leukocytes is withdrawn from said mammal;
- followed by
- (b) extracorporeally reacting the irradiated mixture of leukocytes with a mixture of autologous peptide antigens associated with autoimmune disease allergic reactions, or hypersensitivity disease to form a plurality of peptide antigen-associated leukocytes, wherein the autologous peptide antigens are obtained from said mammal;
- and
- (c) administering the peptide antigen-associated leukocytes to said mammal.
A method for specifically down-regulating the immune system T-cell response of a mammal to an autologous peptide antigen of said mammal, comprising the steps of:
- (a) withdrawing a mixture of leukocytes from said mammal;
- (b) irradiating the mixture of leukocytes in the presence of a psoralen at a temperature between about 20.degree. C. and about 28.degree. C.;
- followed by
- (c) extracorporeally contacting the irradiated mixture of leukocytes with a mixture of autologous peptide antigens associated with autoimmune disease, allergic reactions, or hypersensitivity disease to form a plurality of autologous peptide antigen-associated leukocytes;
- and
- (c) administering the autologous peptide antigen-associated leukocytes to said mammal.
Dependencies are inferred from the claim text.