Random bio-oligomer library, a method of synthesis thereof, and a method of use thereof

A library comprising a multiplicity of solid phase supports, wherein a single peptide species is attached to each solid phase support, and in which the library is prepared by a method comprising repeating k times, wherein k is at least three, the steps of:

• (i) providing at least two aliquots of a solid phase support said supports having an N reactive site;
• (ii) separately introducing a species of subunit of the peptides to each of the aliquots of solid phase supports such that a different subunit is introduced into each aliquot, said subunit having an N-blocking group and at least one of said subunits having a protecting group or a plurality of protecting groups;
• (iii) completely covalently coupling the subunit to substantially all the reactive sites of the solid phase support;
• (iv) thoroughly mixing and removing the N-blocking groups of the aliquots of the solid phase supports whereby an N reactive site is provided;
• and after repeating steps ii through (iv) k times, a final step of removing any protecting groups such that the deprotected peptides remain covalently attached to the solid phase supports.