Viral vectors
AssigneeThe Regents of the University of California
InventorsFlossie Wong-Staal, Michael Mamounas, Eric M. Poeschla, Gunter Kraus, Mark Leavitt
Life of this patent
Estimating the term…
Abstract
Vectors are provided which stably transduce cells, rendering the cells resistant to a target virus. The vectors are amplified upon infection of the cell by a target virus, and spread throughout an infected host in response to infection by the target virus.
Claims21
A vector comprising biologically active nucleic acid sequences from a first and second virus, wherein said nucleic acid sequences of said first virus comprise cis-active AAV nucleic acids for host cell chromosomal integration, said nucleic acid sequences of said second virus comprise a replication defective, rescuable retroviral genome, and wherein said nucleic acid sequences of said second virus also encodes an anti-viral agent operably linked to an expression control sequence.
A mammalian cell transduced with a vector comprising biologically active nucleic acid sequences from a first and second virus, wherein said nucleic acid sequences of said first virus comprise cis-active AAV nucleic acids for host cell chromosomal integration, said nucleic acid sequences of said second virus comprise a replication defective, rescuable retroviral genome, and wherein said nucleic acid sequences of said second virus also encodes an anti-viral agent operably linked to an expression control sequence.
A method for inhibiting viral replication in a cell in vitro, comprising transducing the cell with a vector comprising biologically active nucleic acid sequences from a first and second virus, wherein said nucleic acid sequences of said first virus comprise cis-active AAV nucleic acids for host cell chromosomal integration, said nucleic acid sequences of said second virus comprise a replication defective, rescuable retroviral genome, and wherein said nucleic acid sequences of said second virus also encodes an anti-viral agent operably linked to an expression control sequence.
A method for making anti-viral agents in a cell in vitro, comprising transducing the cell with a vector comprising biologically active nucleic acid sequences from a first and second virus, wherein said nucleic acid sequences of said first virus comprise cis-active nucleic acids encoding viral sequences for host cell chromosomal integration, said nucleic acid sequences of said second virus comprise a replication defective, rescuable viral genome, and wherein said nucleic acid sequences of said second virus encode an anti-viral agent operably linked to an expression control sequence, wherein the cell includes genes necessary for activating said expression control sequence, and culturing the cell under conditions for expression of the anti-viral agent.
Dependencies are inferred from the claim text.