Genetically engineered plant cells and plants exhibiting resistance to glutamine synthetase inhibitors, DNA fragments and recombinants for use in the production of said cells and plants
AssigneePlant Genetic Systems, N.V.
InventorsJan Leemans, Johan Botterman, Charles Thompson, Rao Mouva
Life of this patent
Estimating the term…
Abstract
The invention relates to a DNA fragment containing a determined gene, the expression of which inhibits the antibiotic and herbicidal effects of Bialaphos and related products. It also relates to recombinant vectors, containing such DNA fragment, which enable this protective gene to be introduced and expressed into cells and plant cells.
Claims30
A vector comprising a chimeric gene comprising in sequence:
- (a) a promoter recognized by polymerases of a plant cell;
- and
- (b) a DNA fragment encoding a protein with acetyltransferase activity on a glutamine synthetase inhibitor, wherein said protein is capable of inactivating said glutamine synthetase inhibitor in a plant cell.
A vector comprising a DNA fragment encoding a protein comprising the amino acid sequence:
- __________________________________________________________________________ X Ser Pro Glu Arg Arg Pro Ala Asp Ile Arg Arg Ala Thr Glu Ala Asp MET Pro Ala Val Cys Thr Ile Val Asn His Tyr Ile Glu Thr Ser Thr Val Asn Phe Arg Thr Glu Pro Gln Glu Pro Gln Glu Trp Thr Asp Asp Leu Val Arg Leu Arg Glu Arg Tyr Pro Trp Leu Val Ala Glu Val Asp Gly Glu Val Ala Gly lle Ala Tyr Ala Gly Pro Trp Lys Ala Arg Asn Ala Tyr Asp Trp Thr Ala Glu Ser Thr Val Tyr Val Ser Pro Arg His Gln Arg Thr Gly Leu Gly Ser Thr Leu Tyr Thr His Leu Leu Lys Ser Leu Glu Ala Gln Gly Phe Lys Ser Val Val Ala Val Ile Gly Leu Pro Asn Asp Pro Ser Val Arg Met His Glu Ala Leu Gly Tyr Ala Pro Arg Gly Met Leu Arg Ala Ala Gly Phe Lys His Gly Asn Trp His Asp Val Gly Phe Trp Gln Leu Asp Phe Ser Leu Pro Val Pro Pro Arg Pro Val Leu Pro Val Thr Glu lle __________________________________________________________________________
- in which X is Met or Val, or a part of said protein having phosphinothricin acetyltransferase activity.
A vector comprising a DNA fragment encoding a protein with acetyltransferase activity on a glutamine synthetase inhibitor, wherein said protein is capable of inactivating said glutamine synthetase inhibitor in a plant cell.
Dependencies are inferred from the claim text.