Immortalized dendritic cells
AssigneeZymoGenetics, Inc.
InventorsVivian L. MacKay, Emma E. Moore
Life of this patent
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Abstract
The present invention provides an immature dendritic cell line derived from p53 growth suppressor gene deficient animals. The immature dendritic cell line may be induced to become an activated dendritic cell line that will stimulate T-cells to proliferate. The cell line is useful for presentation of antigens involved in autoimmune disease and analysis of peptides that produce a T-cell response.
Claims15
A dendritic cell line , wherein said cell line is deposited at the ATCC as JAWS II. (ATCC accession # CRL-11904).
A method for in vitro assay of antigen-specific responder cell stimulation comprising:
- activating JAWS II dendritic cells (ATCC # CRL-11904);
- exposing said activated JAWS II dendritic cells to an exogenous antigen, thereby producing antigen-presenting stimulator cells;
- inhibiting proliferation of said antigen-presenting stimulator cells by exposure to .gamma. irradiation or to mitomycin C;
- combining said antigen-presenting stimulator cells with responder cells;
- and
- measuring activation of the responder cells.
A method for obtaining a dendritic cell that expresses a heterologous MHC class II protein at the cell surface comprising:
- transfecting JAWS II dendritic cells (ATCC # CRL-11904) with a polynucleotide encoding a heterologous MHC class II protein;
- and
- selecting a subset of the dendritic cells that expresses the heterologous MHC class II protein at the cell surface, thereby forming selected MHC class II-specific dendritic cells.
Dependencies are inferred from the claim text.