Fusion glycoproteins
AssigneeThe Public Health Research Institute of the City of New York, Inc.
InventorsSamuel Kayman, Abraham Pinter
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Abstract
Novel expression vectors are provided for expressing a fusion glycoprotein. The fusion glycoprotein contains the N-terminal globular domain of a retroviral env surface protein linked to a selected glycopeptide. Truncation glycoproteins as well as insertion glycoproteins are expressed using the vectors.
Claims74
In an expression vector for infecting or transfecting a cell to express a glycosylated protein, the improvement wherein said protein is a fusion glycoprotein comprising (a) an amino acid sequence that has a biological activity and is from a protein that lacks a hydrophobic glycosylation signal located about seven residues N-terminal to a Cys-Trp-leu-cys sequence (Seq. ID. No. 18), said amino acid sequence being operably linked to the C-terminus of (b) a retrovirus env surface protein,
- N-terminal carrier fragment that includes all of the Cys residues of the N-terminal globular domain of said surface protein, and further wherein said env surface protein contains an N-glycan attachment site within a hydrophobic glycosylation signal located about seven residues N-terminal to a Cys-trp-leu-cys sequence (Seq. ID. No. 18).
Dependencies are inferred from the claim text.