Coating composition for culturing animal cells and method for culturing of the cells in serum-free condition
AssigneeKurashiki Boseki Kabushiki Kaisha
InventorsSusumu Katsuen, Kunihiro Ohshima, Seiko Kawamura, Ryohei Yamamoto, Toyokazu Nishino
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Abstract
Disclosed is a coating compositions for culturing animal adhesive cells comprising a water-insoluble polymer dissolved in a lower alcohol or an aqueous lower alcohol which enable to enhance the adhesive ability and growth of the adhesive cells. Also disclosed is serum-free cell culturing method using culture vessels or carrier coated with the water-insoluble polymer having cell adhesive activity on at least a part of the surface which enable to not only culture but also subculture a variety of adhesive cells including vascular endothelial cell under serum-free condition.
Claims20
A serum-free culturing method of animal adhesive cells comprising:
- 1) coating the surface of a culture vessel or a carrier with a water-insoluble polymer dissolved in a lower alcohol or an aqueous lower alcohol;
- wherein the said lower alcohol is selected from methanol, ethanol, propanol and butanol;
- wherein said polymer contains as monomer ingredients at least one monomer chemically bound to a peptide having a cell adhesive activity, said monomer being selected from the group consisting of (metha)acrylic acid, glycidyl (metha)acrylate and N-(metha)acryloyloxy succinimide, wherein the concentration of the water-insoluble polymer in the lower alcohol or aqueous lower alcohol is from about 0.001 to about 0.1% (w/v);
- wherein the amount of the peptide having cell adhesive activity is greater than about 2 .mu.g/mg of the polymer;
- and wherein the polymer has a formulation ratio of hydrophilic monomers to all other monomers in the range from 90:10 to 10:90;
- and 2) culturing the animal adhesive cells in the vessel or carrier using a medium which contains no serum while in contact with the polymer.
Dependencies are inferred from the claim text.