Porphyrin, azaporphyrin, and related fluorescent dyes free of aggregation and serum binding
AssigneeDiatron Corporation
InventorsWalter B. Dandliker, Mao-Lin Hsu
Life of this patent
Estimating the term…
Abstract
Fluorescent dyes which are free of aggregation and serum binding are provided. These dyes are suitable for applications such as fluorescence immunoassays, in vivo imaging and in vivo tumor therapy. The dyes are particularly useful in fluorescence immunoassays of biological samples containing serum. Such dyes have two polyoxyhydrocarbyl moities, one located on either side of a planar molecular structure such as a porphyrin derivative, azaporphyrin derivative, corrin derivative, sapphyrin derivative or porphycene derivative.
Claims27
A detectably labeled marker component which comprises a fluorophore moiety coupled to two solubilizing polyoxyhydrocarbyl moieties, one located on either side of the fluorophore moiety,
- wherein said polyoxyhydrocarbyl moieties are selected from polyethers, polyols and water soluble polymers and wherein said fluorophore moiety is a porphyrin derivative or an azaporphyrin derivative wherein one or more bridging carbon atoms has been replaced by nitrogen.
A detectably labeled marker component which comprises a fluorophore moiety comprising a nitrogen containing macrocyclic ligand with conjugated .pi.-electron system selected from a porphyrin derivative or an azaporphyrin derivative having 1 to 4 bridging carbon atoms replaced by nitrogen, a corrin derivative, a sapphyrin derivative or a porphycene derivative coordinated to a central atom capable of forming octahedral coordination complexes and is selected from silicon, germanium, phosphorous and tin coupled to two solubilizing polyoxyhydrocarbyl moieties, comprising axial ligands which coordinate to the central atom, one linked on either side of the fluorophore moiety wherein said polyoxyhydrocarbyl moities comprise polyethylene glycol or polyethylene glycol derivatives.
A detectably labelled marker component which comprises a fluorophore moiety comprising a macrocyclic multidentate ligand with a conjugated .pi.-electron system coordinated to a central germanium atom capable of forming octahedral coordination complexes coupled to two solubilizing polyoxyhydrocarbyl moities, comprising axial ligands which coordinate to the central atom, one linked on either side of the fluorophore moiety, wherein said polyoxyhyrdocarbyl moieties comprise polyethylene glycol or polyethylene glycol derivatives.
A detectably labelled marker component which comprises a fluorophore moiety coupled to two solubilizing polyoxyhydrocarbyl moieties in which in the presence of serum components in aqueous solution is characterized by transient state fluorescence emission having parallel and perpendicular components of substantially the same intensities as without serum, wherein said polyoxyhydrocarbyl moieties are selected from polyethers, polyols and water soluble polymers.
Dependencies are inferred from the claim text.