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US 5,641,870 AGranted
Low pH hydrophobic interaction chromatography for antibody purification
AssigneeGenentech, Inc.
InventorsErnst H. Rinderknecht, Gerardo A. Zapata
Life of this patent
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Abstract
A process for purifying an antibody is provided. In this process, a mixture containing the antibody and contaminant is subjected to low pH hydrophobic interaction chromatography (LPHIC) optionally at low salt concentration. The antibody is eluted from the column in the fraction which does not bind thereto. This process can be preceded and followed by other purification steps.
Claims16
A process for purifying an antibody comprising loading a mixture containing the antibody on a hydrophobic interaction chromatography column and eluting the antibody from the column with a buffer having a pH of about 2.5 to about 4.5.
Dependencies are inferred from the claim text.