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US 5,641,670 AGranted
Protein production and protein delivery
AssigneeTranskaryotic Therapies, Inc.
InventorsDouglas A. Treco, Michael W. Heartlein, Richard F. Selden
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AssigneeTranskaryotic Therapies, Inc.
InventorsDouglas A. Treco, Michael W. Heartlein, Richard F. Selden
Estimating the term…
The invention relates to constructs comprising: a) a targeting sequence; b) a regulatory sequence; c) an exon; and d) an unpaired splice-donor site. The invention further relates to a method of producing protein in vitro or in vivo comprising the homologous recombination of a construct as described above within a cell. The homologously recombinant cell is then maintained under conditions which will permit transcription and translation, resulting in protein expression. The present invention further relates to homologously recombinant cells, including primary, secondary, or immortalized vertebrate cells, methods of making the cells, methods of homologous recombination to produce fusion genes, methods of altering gene expression in the cells, and methods of making a protein in a cell employing the constructs of the invention.
A homologously recombinant cell having incorporated therein a new transcription unit, wherein the new transcription unit comprises an exogenous regulatory sequence, an exogenous exon and a splice-donor site, operatively linked to the second exon of an endogenous gene, wherein the homologously recombinant cell comprises said exogenous exon in addition to exons present in said endogenous gene.
The DNA plasmid pREPO18.
A homologously recombinant cell comprising the dhfr gene, the neo gene, the CMV promoter, hGH exon 1 and an unpaired splice-donor site targeted to a position upstream of the endogenous erythropoietin regulatory region.
Dependencies are inferred from the claim text.