Process for transforming monocotyledonous plants
AssigneePlant Genetic Systems, N.V.
InventorsKathleen D'Halluin, Elke Gobel
Life of this patent
Estimating the term…
Abstract
A rapid and efficient method for transforming monocotyledonous plants, especially gramineous plants, particularly corn, using either intact tissue capable of forming compact embryogenic callus or compact embryogenic callus obtained from such tissue to obtain transgenic monocotyledonous plants, is provided. Preferably, the tissue or embryogenic callus is wounded and/or degraded prior to transformation. The resulting wounded and/or degraded, intact tissue or compact embryogenic callus is then transformed, preferably by direct gene transfer, e.g., by means of electroporation, with one or more DNA fragments (e.g., foreign DNA fragments).
Claims32
A process for the stable integration of a DNA, said DNA comprising a gene that is functional in a cell of a corn plant in the nuclear genome of a corn plant, said process comprising:
- (a) providing a type I callus,
- (b) wounding and/or degrading said type I callus and transferring said DNA into the nuclear genome of a cell in said type I callus to generate a transformed cell, and,
- (c) regenerating a transformed corn plant from said transformed cell.
A process for the stable integration of a DNA, said DNA comprising a gene that is functional in a cell of a corn plant, into the nuclear genome of a corn plant, said process comprising:
- (a) obtaining an immature embryo from said corn plant,
- (b) wounding and/or degrading said immature embryo by treatment with an enzyme capable of degrading the cell walls of the cells of said immature embryo for a short period of time so as not to cause a complete disruption of the tissues and transferring said DNA into the nuclear genome of a cell in said immature embryo to generate a transformed cell, and,
- (c) regenerating a transformed corn plant from said transformed cell.
Dependencies are inferred from the claim text.