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US 5,641,662 AGranted
Transfection of lung via aerosolized transgene delivery
AssigneeThe Regents of the University of California
InventorsRobert James Debs, Ning Zhu
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Abstract
Methods and compositions for producing a mammal capable of expressing an exogenously supplied gene in cells of the airway are disclosed. Lipid carrier-nucleic acid complexes are prepared then delivered via aerosol to the lung airway. The invention provides a direct method for transforming pulmonary cells as a means for treating disorders of the lung as for providing a means for delivering substances systematically following expression in the lung.
Claims15
A composition comprising:
- a nebulized transfection agent, prepared by nebulizing a mixture comprising complexes between DNA molecules comprising expression cassettes and cationic lipid carriers in a pharmaceutically acceptable carrier, wherein the cationic lipid carriers are in the form of vesicles having diameter of about 100-500 nm, wherein said expression cassettes and said cationic lipid carriers are present in said mixture in a ratio ranging from about 4:1 to about 1:2 mg DNA to micromole of cationic lipid, wherein said mixture is substantially free of macroaggregates of said complexes, and wherein said expression cassettes comprise a DNA sequence that is transcribed to produce a transcription product in vivo in a mammalian lung cell transfected by said nebulized transfection agent.
Dependencies are inferred from the claim text.