.alpha.-l-rhamnosidase for obtaining rhamnose, a process for its preparation and its use
AssigneeHoechst Aktiengesellschaft
InventorsJohannes Meiwes, Dieter Wullbrandt, Carlo Giani
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Abstract
The invention relates to .alpha.-L-rhamnosidase, which catalyzes the cleavage of the bond between terminal rhamnose and the aglycone of rhamnose-containing glycosides, a process for preparing it by biotechnological means, and its use for preparing L(+)-rhamnose (6-deoxy-L-mannose).
Claims16
An isolated .alpha.-L-rhamnosidase that catalyzes the cleavage of the bond between terminal L-rhamnose and the aglycone of a rhamnose-containing glycoside, said .alpha.-L-rhamnosidase having a molecular weight of from 60 to 100 kD and an isoelectric point ascertained by chromatofocussing of from about 5.6 to 5.8.
An isolated .alpha.-L-rhamnosidase that catalyzes the cleavage of the bond between terminal rhamnose and the aglycone of rhamnose-containing glycosides, having a molecular weight of 60-100 kd, which .alpha.-L-rhamnosidase contains the amino-terminal amino add sequence
- Asp Thr Asn Asp Gln Thr Ser Ala Lys Val Asp Arg Gly Thr Phe Asp Asp Pro Ala Ala Arg Leu (SEQ ID NO:
- 1)
- or
- Phe Phe Gly Ser Xaa Gln Ser Leu Tyr Leu Lys Leu Val Leu Lys Phe Gly Thr Leu Phe Asp Xaa Ala (SEQ ID NO:2).
A raw enzyme of .alpha.-L-rhamnosidase produced by a process comprising the steps of:
- a) fermenting Penicillium sp. DSM 6825, Penicillium sp. DSM 6826, or a mutant of Penicillium sp. DSM 6825 or 6826 that catalyzes the cleavage of the bond between terminal L-rhamnose and the aglycone of a rhamnose-containing glycoside;
- b) separating off the biomass from the culture broth;
- and
- c) concentrating the culture supernatant.
Dependencies are inferred from the claim text.