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US 5,641,650 AGranted
Expression of heterologous polypeptides in halobacteria
AssigneeThe Regents of the University of California
InventorsGeorge J. Turner, Mary C. Betlach
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Abstract
This invention relates to the preparation and use of expression systems capable of producing heterologous polypeptides in halobacterial hosts.
Claims13
An expression vector useful for the production of heterologous polypeptide in a halobacterial host comprising:
- a) halobacterial transcription and translation regulatory DNA of the bacteriorhodopsin gene;
- b) DNA encoding a heterologous polypeptide;
- and
- c) DNA encoding transcription and translation stop signals of the bacteriorhodopsin gene;
- wherein said DNA of a), b) and c) is operably linked, and wherein said heterologous polypeptide is other than a halobacteria polypeptide.
2 more elements
A method for producing a heterologous polypeptide in a halobacterial host comprising:
- a) transforming a halobacterial host with an expression vector, said expression vector comprising:
- i) halobacterial bacteriorhodopsin transcription and translation regulatory DNA;
- ii) DNA encoding a heterologous polypeptide;
- and
- iii) DNA encoding halobacterial bacteriorhodopsin transcriptional and translation stop signals;
- iv) wherein said DNA of i), ii), and iii) is operably linked;
- b) transforming a halobacterial host with said expression vector;
- and
- c) causing expression of said DNA encoding the heterologous polypeptide;
- wherein said heterologous polypeptide is other than a halobacteria polypeptide.
7 more elements
Dependencies are inferred from the claim text.