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AssigneeThe Salk Institute for Biological Studies
InventorsRonald M. Evans, Ronald Schule
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Hormone receptors and the transcription factor Jun/AP-1 have been shown to reciprocally repress one another by a mechanism which is independent of DNA binding. For example, over-expression of AP-1 represses glucocorticoid-induced activation of genes carrying a functional glucocorticoid response element. Conversely, glucocorticoid has been shown to repress the transcriptional activation of genes which are controlled by promoters which contain the AP-1 binding site. In addition, methods are disclosed for selecting compounds useful for treating cells undergoing uncontrolled proliferation, such compounds being capable of disrupting the function of AP-1, but display substantially no ability to promote the transcriptional activation of hormone responsive genes.
A method for selecting a compound, said method comprising selecting a compound which disrupts the function of Activator Protein-1 (AP-1), as determined by a first assay system, but does not promote transcriptional activation of a steroid hormone responsive gene, as determined by a second assay system, wherein:
A method for identifying a compound which disrupts an Activator Protein-1 (AP-1) response pathway, but which exerts no substantial effect on asteroid hormone response pathway, said method comprising identifying a compound which has both an inhibitory effect on AP-1-responsive expression, as determined by a first assay system, and no substantial effect on steroid hormone-responsive expression, as determined by a second assay system, wherein:
A method to repress transcription activation of a steroid hormone-responsive gene by steroid hormones in an expression system that expresses a steroid hormone-responsive gene, said method comprising:
A method to repress transcription activation of a steroid hormone-responsive gene by steroid hormone compounds in an expression system that expresses a steroid hormone-responsive gene, said method comprising:
A method to repress transcription activation of an Activator Protein-1 (AP-1)-responsive gene by AP-1 in an expression system that expresses an AP-1-responsive gene, said method comprising:
A method to overcome the repression of expression of a gene product from a gene in an expression system having a steroid hormone present, when said gene is subject to negative regulation by steroid hormone receptors, said method comprising:
A method to overcome the inhibition of proliferation of cultured lymphoid cells by asteroid hormone in the presence of asteroid hormone receptor, said method comprising:
A method for selecting a compound which disrupts the function of Activator Protein-1 (AP-1), said method comprising:
A method for selecting a compound which disrupts the function of Activator Protein-1 (AP-1), but does not affect the transcriptional activation of steroid hormone-responsive genes, said method comprising:
A method to repress transcription activation of an Activator Protein-1 (AP-1)-responsive gene by AP-1 in an expression system that expresses an AP-1-responsive gone, said method comprising:
Dependencies are inferred from the claim text.