Measuring apparatus for measuring an optical property of a fluorescent sample

A measuring apparatus for measuring an optical property of a sample comprising:

• a light source for irradiating the sample with a light including ultraviolet rays;
• UV cut member, movable between a first position and a second position, for cutting off the ultraviolet rays, wherein said UV cut member allows the ultraviolet rays of said light source to irradiate the sample at the first position and prevents the ultraviolet rays from irradiating the sample at the second position;
• a light receiving element for receiving a light from the sample irradiated by said light source;
• a controller for causing said light source to irradiate the sample with said UV cut member at the first position and at the second position;
• a first memory for storing a first output of said light receiving element at irradiation of said light source with said UV cut member at the first position;
• a second memory for storing a second output of said light receiving element at irradiation of said light source with said UV cut member at the second position;
• a means for generating weighting coefficients which weight the first output and second output of said light receiving element respectively;
• and
• a calculator for calculating an optical property of the sample based on the first and second output stored in said first and second memory and the weighting coefficients generated by said generating means.

A method for measuring an optical property of a sample, said method comprising the steps of:

• irradiating the sample with a first light including ultraviolet rays;
• receiving a light from the sample irradiated by the first light and outputting a first signal corresponding to the received light;
• irradiating the sample with a second light, wherein the ratio of ultraviolet rays of the second light is different from that of said first light;
• receiving a light from the sample irradiated by the second light and outputting a second signal corresponding to the received light;
• generating weighting coefficients which weight the first signal and second signal respectively;
• and
• calculating an optical property of the sample based on the first and second signal and the weighting coefficients.

A measuring apparatus for measuring a total spectral radiant factor of a fluorescent sample comprising:

• a first light source for irradiating the sample with a light including ultraviolet rays;
• a second light source for irradiating the sample with a light not including ultraviolet rays;
• a first measuring device for measuring a spectral luminous intensity of a light radiated from the sample irradiated by said first and second light source;
• a second measuring device for measuring a spectral luminous intensity of a light from the first and second light source;
• a controller for controlling said first and second measuring device so as to measure a nonfluorescent white standard sample, whose spectral luminous intensity is known, and to measure a measurement sample in sequence;
• a generating means for generating weighting coefficients which weight each of the spectral luminous intensities measured by said first and second measuring device;
• a calculator for calculating a total spectral radiant factor .beta.t,x (.lambda.) of the sample as follows:
• ##EQU5##
• S'(.lambda.)=a1(.lambda.).multidot.S1(.lambda.)+a2(.lambda.).multidot.S2(.l ambda.)
• R'(.lambda.)=a1(.lambda.).multidot.R1(.lambda.)+a2(.lambda.).multidot.R2(.l ambda.)
• Sw'(.lambda.)=a1(.lambda.).multidot.Sw1(.lambda.)+a2(.lambda.).multidot.Sw2 (.lambda.)
• Rw'(.lambda.)=a1(.lambda.).multidot.Rw1(.lambda.)+a2(.lambda.).multidot.Rw2 (.lambda.)
• where
• W(.lambda.):
• known spectral luminous intensity of the nonfluorescent white standard sample;
• S1(.lambda.):
• measured spectral luminous intensity of the sample irradiated by said first light source;
• S2(.lambda.):
• measured spectral luminous intensity of the sample irradiated by said second light source;
• R1(.lambda.):
• measured spectral luminous intensity of the first light source at the sample measurement;
• R2(.lambda.):
• measured spectral luminous intensity of the second light source at the sample measurement;
• Sw1(.lambda.):
• measured spectral luminous intensity of the nonfluorescent white standard sample irradiated by said first light source;
• Sw2(.lambda.):
• measured spectral luminous intensity of the nonfluorescent white standard sample irradiated by said second light source;
• Rw1(.lambda.):
• measured spectral luminous intensity of the first light source at the nonfluorescent white standard sample measurement;
• Rw2(.lambda.):
• measured spectral luminous intensity of the second light source at the nonfluorescent white standard sample measurement;
• and
• a1(.lambda.), a2(.lambda.):
• weighting coefficients.

A measuring apparatus for measuring an optical property of a sample comprising:

• a first light source for irradiating the sample with a first light including ultraviolet rays;
• a second light source for irradiating the sample with a second light, wherein the ratio of ultraviolet rays of the second light is different from that of said first light;
• and
• a calculator for calculating weighting coefficients which synthesize a composite light source from said first and second light source, wherein a measurement value under illumination of the composite light source matches to a measurement value under illumination of a standard light source.