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US 5,635,615 AGranted
High affinity HIV nucleocapsid nucleic acid ligands
AssigneeNeXstar Pharmaceuticals, Inc.
InventorsPatrick Allen, Larry Gold
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Abstract
Methods are described for the identification and preparation of high-affinity nucleic acid ligands to HIV-1 nucleocapsid. Included in the invention are specific RNA ligands to HIV-1 nucleocapsid identified by the SELEX method and RNA ligands that inhibit the function of HIV-1 nucleocapsid.
Claims7
A purified and isolated non-naturally occurring nucleic acid ligand to HIV-1 nucleocapsid.
A nucleic acid ligand to HIV-1 nucleocapsid identified according to the method comprising:
- a) preparing a candidate mixture of nucleic acids;
- b) contacting the candidate mixture of nucleic acids with HIV-1 nucleocapsid, wherein nucleic acids having an increased affinity to HIV-1 nucleocapsid relative to the candidate mixture may by partitioned from the remainder of the candidate mixture;
- c) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture;
- and
- d) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for nucleic acid sequences with relatively higher affinity and specificity for binding to HIV-1 nucleocapsid, whereby "a nucleic acid ligand" of HIV-1 nucleocapsid may be identified.
2 more elements
Dependencies are inferred from the claim text.