Bifunctional and heteroantibodies specific for the high affinity Fc receptor for immunoglobulin G on human mononuclear phagocytes
AssigneeTrustees of Dartmouth College
InventorsMichael W. Fanger, Paul M. Guyre, Clark L. Anderson
Life of this patent
Estimating the term…
Abstract
Monoclonal antibodies which react specifically to Fc receptor of human effector cells IgG are disclosed. Binding of the antibodies to the receptor is not blocked by human immunoglobulin G. The antibodies are useful for targeting human effector cells (e.g. macrophages) against a target cell (e.g. a cancer cell, an infectious agent, etc.) For this purpose, bifunctional antibodies or heteroantibodies can be constructed containing the binding region derived from an anti-Fc receptor antibody and the binding region of a target-specific antibody. Targeted effector cells can be used to kill target cells by cell mediated antibody dependent cytolysis.
Claims28
A bifunctional antibody or heteroantibody, comprising:
- a. at least one antigen binding region derived from an antibody, which binds specifically to the human 72 kDa high affinity Fc receptor for IqG on monocytes, binding not being blocked by human immunoglobulin G;
- and
- b. at least one antigen binding region specific for a target epitope.
A bifunctional antibody or heteroantibody, comprising:
- a. an antigen binding region derived from an antibody which
- i. binds specifically to an epitope of the 72 kDa high affinity Fc receptor for IgG on human monocytes, the epitope being distinct from the ligand binding site for Fc of the receptor;
- ii. is capable of binding to IgG-occupied Fc receptor;
- and
- iii. is not blocked from binding to the receptor by human IgG;
- and
- b. an antigen binding region derived from an antibody specific for an epitope of a target cell.
A heteroantibody, comprising:
- a. an antibody or antibody binding fragment specific for 72 kDa high affinity Fc receptor for IgG on human monocytes, the binding of which to the human Fc receptor is not blocked by human immunoglobulin G;
- b. an antibody or antibody binding fragment thereof specific for an epitope of a target cell.
A bifunctional antibody or heteroantibody comprising:
- a. at least one antigen binding region derived from mab 32.2 produced by hybridoma cell line ATCC No. HB9469 or subclones thereof;
- and
- b. at least one antigen binding region derived from an antibody specific for an epitope of a target cell.
A heteroantibody, comprising:
- a. an Fab fragment which binds specifically to the human 72 kDa high affinity Fc receptor for IgG on monocytes, the antibody binding site on the receptor being distinct from the ligand binding site for Fc and binding not being blocked by human immunoglobulin G;
- and
- b. an Fab fragment which binds an epitope of a target cell.
Dependencies are inferred from the claim text.