Peptides that bind IL-2 receptors
AssigneeAffymax Technologies N.V.
InventorsRonald W. Barrett, Tania Chernov-Rogan, Ann M. Davis
Life of this patent
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Abstract
Peptides that bind to the Interleukin-2 (IL-2) receptor subunits IL-2R.alpha., IL-2R.beta. and IL-2R.gamma., and inhibit the binding of IL-2 to these important receptors, are described. The invention also includes methods for treating diseases related to the activation of these receptors, as well as the receptors for IL-4, -7 and -13. In addition, the present invention also includes methods for assaying for the presence of these receptors on cell surfaces.
Claims37
A peptide that binds to IL-2R.beta., said peptide comprising the sequence X.sub.18 X.sub.19 CSX.sub.22 X.sub.23 X.sub.24 LGX.sub.27 LC (SEQ ID NO:10) wherein:
- X.sub.18 is selected from the group consisting of I and M;
- X.sub.19 is selected from the group consisting of D and S;
- X.sub.22 is selected from the group consisting of E and Q;
- X.sub.23 is selected from the group consisting of A and R;
- X.sub.24 is selected from the group consisting of A and G;
- and
- X.sub.27 is selected from the group consisting of E and D.
A peptide that binds to IL-2R .beta., said peptide comprising a sequence selected from the group consisting of:
- Ile Asp Cys Gly Val Ala Thr Val Gly Glu Leu Cys (SEQ ID NO:11);
- Ile Asp Cys Ser Glu Ala Ala Leu Gly Glu Leu Cys (SEQ ID NO:12);
- Ile Ser Cys Ser Glu Ala Gly Leu Gly Glu Leu Cys (SEQ ID NO:13);
- Ile Asn Cys Ser Glu Ala Val Ile Gly Gln Leu Cys (SEQ ID NO:14);
- Ile Asp Cys Ser Gln Ala Met Leu Gly Glu Leu Cys (SEQ ID NO:15);
- Ile Asp Cys Ser Asn Ala Val Val Gly Gln Leu Cys (SEQ ID NO:16);
- Ile Asp Cys Ser Glu Ala Val Leu Gly Glu Leu Cys (SEQ ID NO:17);
- Ile Asp Cys Ser Ala Ala Gly Leu Gly Glu Leu Cys (SEQ. ID NO:18);
- Leu Asp Cys Ser Asn Ala Gly Val Gly Asp Leu Cys (SEQ ID NO:20);
- Leu Asp Cys Ser Ile Ala Ala Leu Gly Glu Leu Cys (SEQ ID NO:21);
- Leu Asp Cys Ser Glu Ala Val Leu Gly Glu Leu Cys (SEQ ID NO:22);
- Leu Asp Cys Ser Glu Ala Ile Leu Gly Gln Leu Cys (SEQ ID NO:23);
- Leu Asp Cys His Leu Ala Val Leu Gly Glu Leu Cys (SEQ ID NO:24);
- Leu Asp Cys Gly Glu Ala Ile Leu Gly Glu Leu Cys (SEQ ID NO:25);
- Leu Asp Cys Ser Val Ala Val Leu Gly Glu Leu Cys (SEQ ID NO:26);
- Leu Asp Cys Arg Asp Ala Val Leu Gly Glu Leu Cys (SEQ ID NO:27);
- Met Asp Cys Ser Glu Arg Ala Leu Gly Glu Leu Cys (SEQ ID NO:29);
- Met Asp Cys Ser Gln Ala Ala Leu Gly Asp Leu Cys (SEQ ID NO:30;
- Met Asp Cys Ser Val Ala Val Leu Gly Asp Leu Cys (SEQ ID NO:32);
- Met Asp Cys Arg Glu Ala Ala Leu Gly Glu Leu Cys (SEQ ID NO:33);
- Met Asp Cys Ser Asp Ala Val Leu Gly Asp Leu Cys (SEQ ID NO:34);
- Met Asp Cys Trp Glu Ala Ala Leu Gly Glu Leu Cys (SEQ ID NO:35);
- Met Asp Cys Ser Gln Ala Val Leu Gly Glu Leu Cys (SEQ ID NO:38);
- Met Asp Cys Ser Ile Arg Ala Leu Gly Glu Leu Cys (SEQ ID NO:40);
- Met Asp Ser Ser Gln Ala Ala Leu Gly Glu Leu Cys (SEQ ID NO:41);
- Thr Glu Cys Ser Glu Ala Gly Leu Trp Glu Leu Cys (SEQ ID NO:42);
- Val Asp Cys Ser Glu Ala Val Leu Gly Gln Leu Cys (SEQ ID NO:43);
- Met Asp Cys Ser Glu Ala Val Leu Gly Glu Leu Cys (SEQ ID NO:44);
- and Met Asp Cys Ser Gln Ala Gly Leu Gly Glu Leu Cys (SEQ ID NO:45).
A peptide that binds to IL-2R .beta., said peptide comprising a sequence Ile Asp Cys Ser Glu Ala Ala Leu Gly Thr Leu Cys (SEQ ID NO:19.
A peptide that binds to IL-2R .beta., said peptide comprising a sequence Leu Asp Cys Ser Glu Ala Val Leu Gly His Leu Cys (SEQ ID NO:28).
A peptide that binds to IL-2R .beta., said peptide comprising a sequence;
- Met Asp Cys Ser Gln Ala Gly Leu Cys Glu Leu Cys (SEQ ID NO:31).
A peptide that binds to IL-2R .beta., said peptide comprising a sequence Met Asp Cys His Glu Ala Ala Leu Gly His Leu Cys (SEQ ID NO:36).
A peptide that binds to IL-2R .beta., said peptide comprising a sequence Met Asp Cys Ser Glu Ala Leu Leu Gly Glu Leu Cys (SEQ ID NO:37).
A peptide that binds to IL-2R .beta., said peptide comprising a sequence Met Asp Cys Tyr Asp Ala Arg Leu Gly Asp Leu Cys (SEQ ID NO:39).
Dependencies are inferred from the claim text.