Bone morphogenic protein-5(BMP-5) and DNA encoding same
AssigneeGenetics Institute, Inc.
InventorsJohn M. Wozney, Vicki A. Rosen, Elizabeth A. Wang
Life of this patent
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Abstract
Purified BMP-5 proteins and processes for producing them are disclosed. The proteins may be used in the treatment of bone and/or cartilage defects and in wound healing and related tissue repair.
Claims22
A method for producing a purified bone morphogenic protein-5 (BMP-5) said method comprising the steps of
- (a) culturing in a suitable culture medium cells transformed with a DNA sequence comprising the DNA sequence from nucleotide #699 to #2060 of Table III;
- and
- (b) isolating and purifying said protein from said culture medium.
An isolated DNA sequence encoding a bone morphogenic protein (BMP-5) comprising:
- (a) nucleotide #1647 through #2060 of Table III;
- and
- (b) naturally occurring allelic sequences and equivalent degenerative codon sequences thereof.
A method for producing a bone morphogenic protein-5 (BMP-5) comprising:
- (a) transforming a host cell with a vector comprising nucleotide #1647 to #2060 of Table III;
- (b) culturing said transformed host cell in a suitable culture medium;
- (c) isolating and purifying said BMP-5 from said culture medium.
A method for producing a bone morphogenic protein-5 (BMP-5) comprising:
- (a) transforming a host cell with a vector comprising nucleotide #1665 to #2060 of Table III;
- (b) culturing said transformed host cell in a suitable culture medium;
- (c) isolating and purifying said BMP-5 protein from said culture medium.
A method for producing a bone morphogenic protein-5 (BMP-5) comprising:
- (a) transforming a host cell with a vector comprising nucleotide #1 to #2060 of Table III;
- (b) culturing said transformed host cell in a suitable culture medium;
- (c) isolating and purifying said BMP-5 from said culture medium.
A composition comprising an isolated and purified (1) protein having the amino acid sequence from amino acid #317 (Ala) to amino acid #454 (His) as shown in Table III or naturally occurring allelic sequences thereof, and (2) a carrier or a matrix.
A composition comprising an isolated and purified (1) protein having the amino acid sequence from amino acid #323 (Asn) to amino acid #454 (His) as shown in Table III or naturally occurring allelic sequences thereof, and (2) a carrier or a matrix.
A composition comprising an isolated and purified (1) protein having the amino acid sequence from amino acid #1 (Met) to amino acid #454 (His) as shown in Table III or naturally occurring allelic sequences thereof, and (2) a carrier or a matrix.
A composition comprising (1) an isolated and purified protein dimer with at least one of the subunits having the amino acid sequence from amino acid #317 (Ala) to amino acid #454 (His) of Table III or naturally occurring allelic sequences thereof, and (2) a carrier or a matrix.
A composition comprising (1) an isolated and purified protein dimer with at least one of the subunits having the amino acid sequence from amino acid #323 (Asn) to amino acid #454 (His) of Table III or naturally occurring allelic sequences thereof, and (2) a carrier or a matrix.
A composition comprising (1) an isolated and purified protein dimer with at least one of the subunits having the amino acid sequence from amino acid #1 (Met) to amino acid #454 (His) of Table III or naturally occurring allelic sequences thereof, and (2) a carrier or a matrix.
A composition comprising (1) an isolated and purified protein dimer with at least one of the subunits having the amino acid sequence from amino acid #28 (Gly) to amino acid #454 (His) of Table III or naturally occurring allelic sequences thereof, and (2) a carrier or a matrix.
An isolated DNA sequence encoding a bone morphogenic protein-5 (BMP-5) comprising:
- (a) nucleotide #1665 through #2060 of Table III;
- or
- (b) naturally occurring allelic sequences or equivalent degenerative codon sequences thereof.
An isolated DNA sequence encoding a bone morphogenic protein-5 (BMP-5) comprising:
- (a) nucleotide #1 through #2060 of Table III;
- or
- (b) naturally occurring allelic sequences or equivalent degenerative codon sequences thereof.
An isolated DNA sequence encoding a bone morphogenic protein-5 (BMP-5) comprising:
- (a) nucleotide #699 through #2060 of Table III;
- or
- (b) naturally occurring allelic sequences or equivalent degenerative codon sequences thereof.
Dependencies are inferred from the claim text.