DNA encoding BEHAB, a brain hyaluronan-binding protein, and recombinant expression systems for production of BEHAB polypeptides
AssigneeYale University
InventorsSusan Hockfield, Diane M. Jaworski
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Abstract
A gene encoding mammalian brain enriched hyaluronan binding (BEHAB) protein is isolated and characterized from brain tissue and found to have a high degree of sequence homology to members of the proteoglycan tandem repeat family of hyaluronan binding proteins. Unlike other members of the family, however, the expression of the gene is restricted to the central nervous system. BEHAB is expressed in markedly increased levels in human glioma tissue, so that the polypeptide can be used as a marker for diagnostic purposes.
Claims24
An isolated nucleic acid molecule comprising a sequence selected from the group consisting of:
- (a) the sequence of a genomic DNA clone or a cDNA encoding a brain-enriched hyaluronan-binding (BEHAB) protein, wherein said DNA or cDNA is isolated from a mammalian brain library, and wherein the noncoding strand of said DNA or cDNA hybridizes under stringent conditions with a DNA probe having the sequence shown as nucleotides 251 to 1363 of SEQ ID NO:
- 1 or the sequence shown as nucleotides 270 to 1403 of SEQ ID NO:
- 2;
- (b) a sequence degenerate with the sequence of (a);
- and
- (c) a sequence complementary to the full length of the nucleic acid of (a) or (b).
An isolated DNA molecule comprising the sequence shown as nucleotides 251 to 1363 of SEQ ID NO:
- 1.
An isolated DNA molecule comprising the sequence shown as nucleotides 270 to 1403 of SEQ ID NO:
- 2.
An isolated DNA molecule comprising the sequence shown as nucleotides 1 to 156 of SEQ ID NO:
- 7.
Dependencies are inferred from the claim text.