Method and probes for identifying bacteria found in blood
AssigneeHoffmann-La Roche Inc.
InventorDiane U. Leong
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Abstract
Methods and reagents are provided for detecting polynucleotide sequences in bacteria using probes specific for gram-negative and gram-positive bacteria and other specific bacterial species or groups of species respectively. Also provided are methods of amplification using primers specific for bacterial species.
Claims5
A method for determining the presence of a gram negative bacterial polynucleotide in a sample suspected of containing said gram negative bacterial polynucleotide, wherein said bacterial polynucleotide comprises a selected target region, said method comprising:
- (a) amplifying the target region, if any, to a detectable level;
- (b) incubating the amplified target region, if any, with a polynucleotide probe consisting of a nucleotide sequence selected from the group consisting of
- 5'-GACGTAAGGGCCATGATGACTTGACGTC-3'(SEQ ID No. 5), the sequence complementary to SEQ ID No.5,
- 5'-GACGTAAGGGCCATGAGGACTTGACGTC-3'(SEQ ID No. 7), and the sequence complementary to SEQ ID No. 7, under conditions which allow hybridization of the probe to the amplified target region;
- and
- (c) detecting hybrids formed between the amplified target region, if any, and the polynucleotide probe.
A composition for detecting gram negative bacterial species comprising a polynucleotide hybridization assay probe consisting of the sequence
- 5'-GACGTAAGGGCCATGATGACTTGACGTC-3'(SEQ ID No. 5), or its complementary sequence.
A composition for detecting gram negative bacterial species comprising a polynucleotide hybridization assay probe consisting of the sequence
- 5'-GACGTAAGGGCCATGAGGACTTGACGTC-3'(SEQ ID No. 7), or its complementary sequence.
A PCR kit for the detection of gram negative bacterial species comprising a pair of polynucleotide probes, wherein a first probe consists of the sequence
- 5'-GACGTAAGGGCCATGATGACTTGACGTC-3'(SEQ ID No. 5) or a sequence complementary thereto, and a second probe consists of the sequence 5'-GACGTAAGGGCCATGAGGACTTGACGTC-3'(SEQ ID No. 7) or a sequence complementary thereto.
Dependencies are inferred from the claim text.