Method for the diagnosis of HIV using antibodies to prosomes
AssigneeAkzo Nobel N.V.
InventorsKlaus Scherrer, Jean P. Bureau, Faycal Bey
Life of this patent
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Abstract
This invention is directed to a method useful in the diagnosis of HIV. The method involves reacting monoclonal antibodies to particular prosomal proteins with certain blood cell types obtained from a sample, and observing an increase or decrease in the levels of the prosomal proteins on the cell's surface.
Claims6
A method useful in aiding in the diagnosis of human immunodeficiency virus infection, comprising:
- (a) identifying or isolating B4 cells from a blood sample using antibodies to B4 cells;
- (b) incubating the cells obtained in step (a) with a labeled monoclonal antibody to the prosomal protein p23K, and obtaining a quantitative determination of the amount or percentage of cells displaying the p23K protein on their surface;
- and
- (c) comparing the amount or percentage thus obtained with a standard from a person not infected with human immunodeficiency virus,
- whereby a decrease in the amount or percentage of cells displaying the p23K protein is indicative of HIV infection.
A method useful in aiding in the diagnosis of human immunodeficiency virus infection, comprising:
- (a) identifying or isolating T4 cells from a blood sample using antibodies to T4 cells;
- (b) incubating the cells obtained in step (a) with labeled monoclonal antibodies to the prosomal proteins, p23K, p27K, p29K and p30-33K, and obtaining a quantitative determination of the amount or percentage of cells displaying the p23K, p27K, p29K and p30-33K proteins on their surface;
- and
- (c) comparing the amount or percentage thus obtained with a standard from a person not infected with human immunodeficiency virus,
- whereby an increase in the amount or percentage of cells displaying the p23K, p27K and page proteins on their surface, and a decrease in the percentage of cells displaying p30-33K protein on their surface, is indicative of HIV infection.
A method useful in aiding in the diagnosis of human immunodeficiency virus infection, comprising:
- (a) identifying or isolating natural killer (NK) cells from a blood sample using antibodies to NK cells;
- (b) incubating the cells obtained in step (a) with labeled monoclonal antibodies to the prosomal proteins p21K, p25K, p29K, p30-33K and p31K, and obtaining a quantitative determination of the amount or percentage of cells displaying the p21K, p25K, p29K, p30-33K and p31K proteins on their surface;
- and
- (c) comparing the amount or percentage thus obtained with a standard from a person not infected with human immunodeficiency virus,
- whereby an increase in the percentage of cells displaying p21K, p25K, p30-33K and p31K proteins on their surface, and a decrease in the percentage of cells displaying p29K on their surface, is indicative of HIV infection.
A method useful in aiding in the diagnosis of human immunodeficiency virus infection, comprising:
- (a) identifying or isolating T3 cells from a blood sample using antibodies to T3 cells;
- (b) incubating the cells obtained in step (a) with labeled monoclonal antibodies to the prosomal proteins, p21K, p23K, p29K, p30-33K and p31K, and obtaining a quantitative determination of the amount or percentage of cells displaying the p21K, p23K, p29K, p30-33K and p31K proteins on their surface;
- and
- (c) comparing the amount or percentage thus obtained with a standard from a person not infected with human immunodeficiency virus,
- whereby a decrease in the percentage of cells displaying p21K, p23K, p28K, p30-33K and p31K proteins on their surface is indicative of HIV infection.
A method useful in aiding in the diagnosis of human immunodeficiency virus infection, comprising:
- (a) identifying or isolating T8 cells from a blood sample using antibodies to T8 cells;
- (b) incubating the cells obtained in step (a) with labeled monoclonal antibodies to the prosomal proteins, p27K and p30-33K, and obtaining a quantitative determination of the amount or percentage of cells displaying the p27K and p30-33K proteins on their surface;
- and
- (c) comparing the amount or percentage thus obtained with a standard from a person not infected with human immunodeficiency virus, whereby a decrease in the percentage of cells displaying p27K and p30-33K proteins on their surface is indicative of HIV infection.
A diagnostic test kit for use in aiding in the diagnosis of a human immunodeficiency virus infection comprising:
- antibodies directed against cells selected from the group consisting of B4 cells, T4 cells, T8 cells, natural killer cells, T3 lymphocytes, and mixtures of these cells associated with a solid surface;
- and a diagnostic fluid containing labeled antibodies against prosomal proteins selected from the group consisting of p21K, p23K, p25K, p27K, p29K, p30-33K, and p31K.
Dependencies are inferred from the claim text.