Method of detecting ligand interactions
AssigneeGenetics Institute, Inc.
InventorsJohn M. McCoy, Zhijian Lu
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Abstract
Provided by the present invention are novel methods of detecting ligand interactions, as well as regents useful in the method, including DNA and host cells; and more specifically relates to novel methods for the detection of protein/protein interactions and their application in epitope mapping and the study of ligand/receptor interactions. Also provided are vaccines and kits comprising the expression products and host cells of the invention.
Claims28
A DNA comprising a first DNA encoding a thioredoxin-like sequence fused in-frame to a second DNA encoding a flagellin-like sequence,
- wherein said thioredoxin-like sequence is inserted into a domain of said flagellin-like sequence which is dispensable for flagellar function, and
- wherein said DNA encodes a protein which assembles into an exterior organelle.
A DNA comprising the sequence of SEQ ID NO:76 (FIG. 1).
A DNA comprising a DNA encoding SEQ ID NO:77 (FIG. 1).
A DNA, free of homologous chromosomal DNA, comprising a DNA encoding a member selected from the group consisting of SEQ ID NOS:
- 6-29, 31-40, 42-47, 49-63 and 65-71.
A peptide comprising an amino acid sequence selected form the group consisting of SEQ ID NOS:
- 6-29 and 31-40.
Dependencies are inferred from the claim text.