Method for drying polyacrylamide gel after electrophoresis
AssigneeDaiichi Pure Chemicals Co., Ltd.
InventorsKazuaki Notsu, Mieko Shiratori
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Abstract
A polyacrylamide gel which has undergone electrophoresis is dried in the presence of pressure applied via a semipermeable film placed on the gel. Before applying the semipermeable film onto the gel, solution components contained in the gel is replaced by an aqueous solution containing one or more substances selected from the group consisting of saccharides, sugar alcohols having 4 or more carbon atoms, and water-soluble polymers. According to this method, a polyacrylamide gel which has undergone electrophoresis can be dried with ease and reduced costs while maintaining its transparency and without causing cracks even in the case of a gel containing polyacrylamide gel at a high concentration.
Claims7
A method for drying, while applying a pressure using a semipermeable film, a polyacrylamide gel which has been used for electrophoresis, comprising the steps of:
- replacing solution components contained in the gel by an aqueous solution containing one or more substances selected from the group consisting of saccharides, sugar alcohols having 4 or more carbon atoms, and water-soluble polymers, and subsequently,
- applying a semipermeable film onto the polyacrylamide gel with pressure, thereby drying the gel.
Dependencies are inferred from the claim text.