Chimeric gene for the transformation of plants
AssigneeRhone-Poulenc Agrochimie
InventorsMichel Lebrun, Bernard Leroux, Alain Sailland
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Abstract
Chimeric gene for conferring to plants an increased tolerance to a herbicide having as its target EPSPS comprises, in the direction of transcription, a promoter region, a transit peptide region, a coding sequence for glyphosate tolerance and a polyandenylation signal region, wherein the transit peptide region comprises, in the direction of translation, at least one transit peptide of a plant gene encoding a plastid-localized enzyme and then a second transit peptide of a plant gene encoding a plastid-localized enzyme. Production of glyphosate-tolerant plants is disclosed.
Claims20
A chimeric gene for conferring to plants an increased tolerance to glyphosate comprising, in the direction of transcription, a promoter region, a DNA sequence encoding a first transit peptide from a ribulose-1,5,-bisphosphate carboxylase small subunit, a DNA sequence encoding an N-terminal domain of a mature ribulose-1,5-bisphosphate carboxylase small subunit, a DNA sequence encoding a second transit peptide from a ribulose-1,5,-bisphosphate carboxylase small subunit, coding sequence for 5-(enolpyruvyl)shikimate-3-phosphate synthase and an untranslated polyadenylation signal.
Dependencies are inferred from the claim text.