Plant tissue comprising a subgenomic promoter
AssigneeMycogen Plant Science, Inc.
InventorsPaul G. Ahlquist, Roy C. French
Life of this patent
Estimating the term…
Abstract
A subgenomic promoter of a positive strand RNA virus is disclosed which directs the amplified expression of a structural gene in plant tissue. The core region and an upstream activating domain of the subgenomic promoter are identified. This promoter can be utilized in a modified virus, or in an appropriate engineered recombinant DNA derivative which may be chromosomally integrated or maintained as an episome in transformed cells.
Claims6
Plant tissue comprising a plant cell transformed to comprise a DNA segment comprising the nucleotide sequence of a (+) strand plant RNA virus subgenomic core promoter selected from the group consisting of tobamoviruses, tobraviruses, bromoviruses, cucumoviruses and alfalfa mosaic virus, wherein said (+) strand RNA virus comprises a consensus sequence selected from the group consisting of AUCUAUGUU and sequences having a single mismatch thereto, and further comprising an undeviating domain corresponding to the nucleotide sequence from a BstXI site to a BglII site of the cDNA corresponding to the RNA of FIG. 2, and wherein said activating domain is positioned 5' to said subgenomic core promoter sequence, and comprising a plant-expressible structural gene 3' to said subgenomic core promoter sequence, said plant-expressible structural gene being heterologous to the native genes of said plant tissue, and wherein said plant-expressible structural gene is expressed.
Dependencies are inferred from the claim text.