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US 5,633,444 AGranted
Sulfonamide resistance genes and their use
AssigneeRhone-Poulenc Agrochimie
InventorsJean F. Guerineau, Philip M. Mullineaux, Edgar W. Parnell
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AssigneeRhone-Poulenc Agrochimie
InventorsJean F. Guerineau, Philip M. Mullineaux, Edgar W. Parnell
Estimating the term…
Plant cells are transformed by: (i) transforming a plant cell whose growth is sensitive to inhibition by a sulfonamide or a salt thereof with a chimaeric gene comprising (a) a plant promoter, (b) a sulfonamide resistance gene having a sequence encoding a transit peptide fused to the 5'-end of the resistance gene and (c) a plant polyadenylation/terminator sequence; (ii) selecting a transformed plant cell whose growth is resistant to inhibition by a sulfonamide or salt thereof; (iii) optionally, regenerating from the transformed plant cell a genetically transformed plant which exhibit the said resistance; (iv) optionally, obtaining seed from the regenerated plant; and (v) optionally, propagating plants from the seed. The growth of weeds can be controlled by a locus where a transgenic plant obtained as above is being cultivated by applying to the locus an effective amount of a herbicide, such as asulam, which acts by inhibiting dihydropteroate synthase. The sulfonamide resistance gene can be also used as a selectable marker. The sequence of a sulfonamide resistance gene is presented.
A plant cell whose growth is resistant to inhibition by a sulfonamide or a salt thereof, which plant cell has been obtained by:
A transgenic plant exhibiting resistance to a sulfonamide or a salt thereof, which plant contains in its cells a chimaeric gene comprising (a) a plant promoter (b) a sul gene which encodes a modified DHPS conferring sulfonamide resistance, or a sequence having at least 70% homology thereto conferring sulfonamide resistance and which has a sequence encoding a transit peptide cleavable from the modified DHPS fused to the 5'-end of the resistance gene and (c) a plant polyadenylation/terminator sequence wherein (a), (b) and (c) are operably linked.
A transgenic tobacco plant exhibiting resistance to a sulfonamide or a salt thereof which tobacco plant contains in its cells a chimaeric gene comprising (a) a plant promoter (b) a sul gene which encodes a modified DHPS conferring sulfonamide resistance or a sequence having at least 70% homology thereto, and which has a sequence encoding a transit peptide cleavable from the modified DHPS fused to the 5'-end of the resistance gene and (c) a plant polyadenylation/terminator sequence, wherein (a), (b) and (c) are operably linked.
A transgenic oilseed rape plane exhibiting resistance to a sulfonamide or a salt thereof which oilseed rape plant contains in its cells a chimaeric gene comprising (a) a plant promoter (b) a sul gene which encodes a modified DHPS conferring sulfonamide resistance or a sequence having at least 70% homology thereto, and which has a sequence encoding a transit peptide cleavable from the modified DHPS fused to the 5'-end of the resistance gene and (c) a plant polyadenylation/terminator sequence, wherein (a), (b) and (c) are operably linked.
Dependencies are inferred from the claim text.