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US 5,633,442 AGranted
Method of producing pathogen-resistant plants
AssigneeMax-Planck-Gesellschaft zur Forderung der Wissenschaften e.V.
InventorsJeff Schell, Jurgen Logemann, Guido Jach, John Mundy
Life of this patent
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Abstract
Described are a method of producing pathogen-resistant plants in which a protein-synthesis inhibitor gene or a fusion product of the protein-synthesis inhibitor gene or of the protein-synthesis inhibitor protein with ligands permitting specific attachment to cells is introduced into the genotype of plants under the control of an active promotor, and the use of the protein-synthesis inhibitor protein obtained by introducing the protein-synthesis inhibitor gene into the bacterial overproducers for making pharmaceutical preparations.
Claims6
A method of producing fungus-resistant plants comprising introducing a protein-synthesis inhibitor gene or a fusion product of the protein-synthesis inhibitor gene into the genotype of plants under the control of an active promoter, wherein the protein-synthesis inhibitor gene comprises the following DNA sequence:
- ______________________________________ SEQUENCE DESCRIPTION:
- SEQ ID NO. 1 ______________________________________ CTTAATAGCA CATCTTGTCC GTCTTAGCTT TGCATTACAT CC ATG GCG GCA AAG ATG GCG 60 Met Ala Ala Lys Met Ala -1 1 5 AAG AAC GTG GAC AAG CCG CTC TTC Lys Asn Val Asp Lys Pro Leu Phe 10 ACC GCG ACG TTC AAC GTC CAG GCC 108 Thr Ala Thr Phe Asn Val Gln Ala 15 20 AGC TCC GCC GAC TAC GCC ACC TTC Ser Ser Ala Asp Tyr Ala Thr Phe 25 ATC GCC GGC ATC CGC AAC AAG CTC 156 Ile Ala Gly Ile Arg Asn Lys Leu 30 35 CGC AAC CCG GCG CAC TTC TCC CAC Arg Asn Pro Ala His Phe Ser His 45 AAC CGC CCC GTG CTG CCG CCG GTC 204 Asn Arg Pro Val Leu Pro Pro Val 50 GAG CCC AAC GTC CCG CCG AGC AGG Glu Pro Asn Val Pro Pro Ser Arg 55 60 TGG TTC CAC GTC GTG CTC AAG GCC 252 Trp Phe His Val Val Leu Lys Ala 65 TCG CCG ACC AGC GCC GGG CTC ACG Ser Pro Thr Ser Ala Gly Leu Thr 70 75 CTG GCC ATT CGG GCG GAC AAC ATC 300 Leu Ala Ile Arg Ala Asp Asn Ile 80 85 TAC CTG GAG GGC TTC AAG AGC AGC Tyr Leu Glu Gly Phe Lys Ser Ser 90 GAC GGC ACC TGG TGG GAG CTC ACC 348 Asp Gly Thr Trp Trp Glu Leu Thr 100 CCG GGC CTC ATC CCC GGC GCC ACC Pro Gly Leu Ile Pro Gly Ala Thr 105 TAC GTC GGG TTC GGC GGC ACC TAC 396 Tyr Val Gly Phe Gly Gly Thr Tyr 110 115 CGC GAC CTC CTC GGC GAC ACC GAC Arg Asp Leu Leu Gly Asp Thr Asp 120 125 AAG CTG ACC AAC GTC GCT CTC GGC 444 Lys Leu Thr Asn Val Ala Leu Gly 130 CGG CAG CAG CTG GCG GAC GCG GTG Arg Gln Gln Leu Ala Asp Ala Val 135 140 ACC GCC CTC CAC GGG CGC ACC AAG 492 Thr Ala Leu His Gly Arg Thr Lys 145 GCC GAC AAG CCG TCC GGC CCG AAG Ala Asp Lys Pro Ser Gly Pro Lys 150 155 CAG CAG CAG GCG AGG GAG GCG GTG 540 Gln Gln Gln Ala Arg Glu Ala Val 160 165 ACG ACG CTG CTC CTC ATG GTG AAC Thr Thr Leu Leu Leu Met Val Asn 170 GAG GCC ACG CGG TTC CAG ACG GTG 588 Glu Ala Thr Arg Phe Gln Thr Val 175 180 TCT GGG TTC GTG GCC GGG TTG CTG Ser Gly Phe Val Ala Gly Leu Leu 185 CAC CCC AAG GCG GTG GAG AAG AAG 636 His Pro Lys Ala Val Glu Lys Lys 190 195 AGC GGG AAG ATC GGC AAT GAG ATG Ser Gly Lys Ile Gly Asn Glu Met 200 205 AAG GCC CAG GTG AAC GGG TGG CAG 684 Lys Ala Gln Val Asn Gly Trp Gln 210 GAC CTG TCC GCG GCG CTG CTG AAG Asp Leu Ser Ala Ala Leu Leu Lys 215 220 ACG GAC GTG AAG CCT CCG CCG GGA 732 Thr Asp Val Lys Pro Pro Pro Gly 225 AAG TCG CCA GCG AAG TTC GCG CCG Lys Ser Pro Ala Lys Phe Ala Pro 230 235 ATC GAG AAG ATG GGC GTG AGG ACG 780 Ile Glu Lys Met Gly Val Arg Thr 240 245 GCT GTA CAG GCC GCC AAC ACG CTG Ala Val Gln Ala Ala Asn Thr Leu 250 GGG ATC CTG CTG TTC GTG GAG GTG 828 Gly Ile Leu Leu Phe Val Glu Val 255 260 CCG GGT GGG TTG ACG GTG GCC AAG Pro Gly Gly Leu Thr Val Ala Lys 265 GCG CTG GAG CTG TTC CAT GCG AGT 876 Ala Leu Glu Leu Phe His Ala Ser 270 275 GGT GGG AAA TAGGTAGTTT TCCAGGTATA Gly Gly Lys 280 CCTGCATGGG TAGTGTAAAA GTCGAATAAA 935 CATGTCACAG AGTGACGGAC TGATATAAAT AAATAAATAA ACGTGTCACA GAGTTACATA 995 TAAACAAATA AATAAATAAT TAAAAATGTC CAGTTTA 1032. ______________________________________
A method of preparing an agricultural preparation for inducing resistance of plants against fungi comprising the step of introducing a protein-synthesis inhibitor gene into a bacterial overproducer, wherein the protein-synthesis inhibitor gene comprises the following DNA sequence:
- ______________________________________ SEQUENCE DESCRIPTION:
- SEQ ID NO. 1 ______________________________________ CTTAATAGCA CATCTTGTCC GTCTTAGCTT TGCATTACAT CC ATG GCG GCA AAG ATG GCG 60 Met Ala Ala Lys Met Ala -1 1 5 AAG AAC GTG GAC AAG CCG CTC TTC Lys Asn Val Asp Lys Pro Leu Phe 10 ACC GCG ACG TTC AAC GTC CAG GCC 108 Thr Ala Thr Phe Asn Val Gln Ala 15 20 AGC TCC GCC GAC TAC GCC ACC TTC Ser Ser Ala Asp Tyr Ala Thr Phe 25 ATC GCC GGC ATC CGC AAC AAG CTC 156 Ile Ala Gly Ile Arg Asn Lys Leu 30 35 CGC AAC CCG GCG CAC TTC TCC CAC Arg Asn Pro Ala His Phe Ser His 45 AAC CGC CCC GTG CTG CCG CCG GTC 204 Asn Arg Pro Val Leu Pro Pro Val 50 GAG CCC AAC GTC CCG CCG AGC AGG Glu Pro Asn Val Pro Pro Ser Arg 55 60 TGG TTC CAC GTC GTG CTC AAG GCC 252 Trp Phe His Val Val Leu Lys Ala 65 TCG CCG ACC AGC GCC GGG CTC ACG Ser Pro Thr Ser Ala Gly Leu Thr 70 75 CTG GCC ATT CGG GCG GAC AAC ATC 300 Leu Ala Ile Arg Ala Asp Asn Ile 80 85 TAC CTG GAG GGC TTC AAG AGC AGC Tyr Leu Glu Gly Phe Lys Ser Ser 90 GAC GGC ACC TGG TGG GAG CTC ACC 348 Asp Gly Thr Trp Trp Glu Leu Thr 100 CCG GGC CTC ATC CCC GGC GCC ACC Pro Gly Leu Ile Pro Gly Ala Thr 105 TAC GTC GGG TTC GGC GGC ACC TAC 396 Tyr Val Gly Phe Gly Gly Thr Tyr 110 115 CGC GAC CTC CTC GGC GAC ACC GAC Arg Asp Leu Leu Gly Asp Thr Asp 120 125 AAG CTG ACC AAC GTC GCT CTC GGC 444 Lys Leu Thr Asn Val Ala Leu Gly 130 CGG CAG CAG CTG GCG GAC GCG GTG Arg Gln Gln Leu Ala Asp Ala Val 135 140 ACC GCC CTC CAC GGG CGC ACC AAG 492 Thr Ala Leu His Gly Arg Thr Lys 145 GCC GAC AAG CCG TCC GGC CCG AAG Ala Asp Lys Pro Ser Gly Pro Lys 150 155 CAG CAG CAG GCG AGG GAG GCG GTG 540 Gln Gln Gln Ala Arg Glu Ala Val 160 165 ACG ACG CTG CTC CTC ATG GTG AAC Thr Thr Leu Leu Leu Met Val Asn 170 GAG GCC ACG CGG TTC CAG ACG GTG 588 Glu Ala Thr Arg Phe Gln Thr Val 175 180 TCT GGG TTC GTG GCC GGG TTG CTG Ser Gly Phe Val Ala Gly Leu Leu 185 CAC CCC AAG GCG GTG GAG AAG AAG 636 His Pro Lys Ala Val Glu Lys Lys 190 195 AGC GGG AAG ATC GGC AAT GAG ATG Ser Gly Lys Ile Gly Asn Glu Met 200 205 AAG GCC CAG GTG AAC GGG TGG CAG 684 Lys Ala Gln Val Asn Gly Trp Gln 210 GAC CTG TCC GCG GCG CTG CTG AAG Asp Leu Ser Ala Ala Leu Leu Lys 215 220 ACG GAC GTG AAG CCT CCG CCG GGA 732 Thr Asp Val Lys Pro Pro Pro Gly 225 AAG TCG CCA GCG AAG TTC GCG CCG Lys Ser Pro Ala Lys Phe Ala Pro 230 235 ATC GAG AAG ATG GGC GTG AGG ACG 780 Ile Glu Lys Met Gly Val Arg Thr 240 245 GCT GTA CAG GCC GCC AAC ACG CTG Ala Val Gln Ala Ala Asn Thr Leu 250 GGG ATC CTG CTG TTC GTG GAG GTG 828 Gly Ile Leu Leu Phe Val Glu Val 255 260 CCG GGT GGG TTG ACG GTG GCC AAG Pro Gly Gly Leu Thr Val Ala Lys 265 GCG CTG GAG CTG TTC CAT GCG AGT 876 Ala Leu Glu Leu Phe His Ala Ser 270 275 GGT GGG AAA TAGGTAGTTT TCCAGGTATA Gly Gly Lys 280 CCTGCATGGG TAGTGTAAAA GTCGAATAAA 935 CATGTCACAG AGTGACGGAC TGATATAAAT AAATAAATAA ACGTGTCACA GAGTTACATA 995 TAAACAAATA AATAAATAAT TAAAAATGTC CAGTTTA 1032. ______________________________________
Dependencies are inferred from the claim text.