Feedcrops enriched in sulfur amino acids and methods for improvements
AssigneeE. I. Du Pont de Nemours and Company
InventorChristine I. Wandelt
Life of this patent
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Abstract
There is provided a chimeric gene and a method to increase the seed methionine content in plants. The chimeric gene is capable of transforming plants, particularly rapeseed and soybean, to overexpress a methionine-rich maize seed storage protein in seeds. There is also provided the plants and seeds containing the chimeric gene.
Claims16
A chimeric gene comprising the following elements operably linked in a 5' to 3' orientation;
- (a) a dicotyledonous seed-specific promoter, (b) an intracellular localization sequence, (c) the maize 10 kD zein coding region, and (d) a 3' non-coding sequence, wherein said chimeric gene causes an increase in total seed methionine in plant cells in which the gene is expressed as compared to untransformed plant cells.
A dicotyledonous plant transformed with a chimeric gene comprising the following elements operably linked in a 5' to 3' orientation;
- (a) a dicotyledonous seed-specific promoter, (b) an intracellular localization sequence, (c) the maize 10 kD zein coding region, and (d) a 3' non-coding sequence, wherein said chimeric gene causes an increase in total seed methionine in plant cells in which the gene is expressed as compared to untransformed plant cells.
A method of increasing the sulfur amino acid content of a dicotyledonous plant comprising:
- (a) transforming a dicotyledonous plant cell with a chimeric gene comprising the following elements operably linked in a 5' to 3' orientation;
- (a) a dicotyledonous seed-specific promoter, (b) an intracellular localization sequence, (c) the maize 10kD zein coding region, and (d) a 3' non-coding sequence;
- (b) selecting transformed plant cells from step (a) on the basis of a selectable marker;
- (c) growing fertile, sexually mature plants from the selected transformed plant cell of step (b);
- and
- (d) selecting progeny seed from the fertile plants of step (b) for increased levels of total seed methionine relative to dicotyledonous plant cells of the same type as those transformed in step (a) but not containing the chimeric gene.
A method of increasing the methionine content of soybean or rapeseed plants comprising:
- (a) transforming a soybean or rapeseed plant cell with a chimeric gene comprising the following elements operably linked in a 5' to 3' orientation;
- (a) a dicotyledonous seed-specific promoter, (b) an intracellular localization sequence, (c) the maize 10kD zein coding region, and (d) a 3' non-coding sequence;
- (b) selecting transformed plant cells from step (a) on the basis of a selectable marker;
- (c) growing fertile, sexually mature plants from the selected transformed plant cell of step (b);
- and
- (d) selecting progeny seed from the fertile plants of step (b) for increased levels of total seed methionine relative to soybean or rapeseed plant cells of the same type as those transformed in step (a) but not containing the chimeric gene.
Dependencies are inferred from the claim text.