Production of 1,3-propanediol from glycerol by recombinant bacteria expressing recombinant diol dehydratase
AssigneeE. I. Du Pont de Nemours and Company
InventorsVasantha Nagarajan, Charles E. Nakamura
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Abstract
A process is provided for the bioconversion of glycerol to 1,3-propanediol in which genes from a bacteria known to possess a diol dehydratase enzyme for 1,2-propanediol degradation are cloned into a bacterial host and the host is grown in the presence of glycerol; expression of the foreign genes in the host cell facilitates the enzymatic conversion of glycerol to 1,3-propanediol which is isolated from the culture.
Claims10
A cosmid comprising a DNA fragment of about 35 kb isolated from Klebsiella pneumoniae wherein said fragment encodes an active diol dehydratase enzyme having the restriction digest in FIG. 5, columns numbered 4, said cosmid contained within a transformed E. coli deposited with the American Type Culture Collection under accession number ATCC 69790.
A isolated gene encoding an active diol dehydratase enzyme comprising a contiguous sequence which consists of SEQ ID NO:
- 1.
A isolated gene encoding an active alcohol dehydrogenase comprising a contiguous sequence which consists of SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.