Method for the isolation and purification of vitamin K-dependent proteins
AssigneeImmuno Aktiengesellschaft
InventorsBernhard Fischer, Artur Mitterer, Friedrich Dorner
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Abstract
A method for the separation of vitamin K-dependent proteins from non-vitamin K-dependent accompanying proteins is described wherein the method is characterized in that at least anion exchange chromatography and optionally affinity chromatography is carried out as well. The method is suitable especially for the purification of Factor II, VII, IX, X as well as Protein S, Protein C and Protein Z. With the aid of the method according to the invention a vitamin K-dependent protein is obtained which is present at a purity of 95%.
Claims17
Method for the separation of a vitamin K-dependent protein from non-vitamin K-dependent accompanying proteins from a protein-containing solution, characterized in that calcium ions are added to this solution and the calcium-containing solution is brought in contact with an anion exchanger under conditions in which the accompanying proteins, but not the vitamin K-dependent protein, are adsorbed.
Highly purified vitamin K-dependent protein having a purity of at least 95%, wherein said highly purified vitamin K-dependent protein is obtainable by the steps of (A) adding calcium ions to a solution containing a vitamin K-dependent protein and non-vitamin K-dependent proteins, and (B) contacting the solution resulting from step (A) with an anion exchanger under conditions wherein only the non-vitamin K-dependent proteins are adsorbed to the anion exchanger, thereby maintaining original conformation and stability of said vitamin K-dependent protein.
A method for separating vitamin K-dependent proteins from non-vitamin K-dependent proteins, comprising the steps of:
- (a) adding calcium to a solution containing the vitamin K-dependent proteins and the non-vitamin K-dependent proteins;
- (b) contacting the solution of step (a) with an anion exchanger to selectively adsorb the non-vitamin K-dependent proteins, thereby separating the non-vitamin K-dependent proteins from the vitamin K-dependent proteins.
Dependencies are inferred from the claim text.