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AssigneesBayer Aktiengesellschaft, Mt. Sinai School of Medicine of the City Univ. of New York
InventorsSophie S. Fan, Daniel Ben-David, Albert Cupo, Gena Fischer, Grace E. Martin, Leonard Ornstein, Gregory M. Colella
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Methods for blood sample preparation using reagent compositions which include a zwitterionic surfactant for sphering of cells to eliminate orientational noise. When the reagent composition including a dye for staining a subset of cells is reacted with a blood sample, and the reaction mixture is passed through the sensing region of a flow cytometer, the light scattered and absorbed by each cell is measured, the stained cells can be distinguished from the unstained cells, and when the cells are reticulocytes and mature erythrocytes, respectively, the volume and hemoglobin concentration of each reticulocyte or erythrocyte, and the hemoglobin content, mean cell volume, mean corpuscular hemoglobin concentration, and mean cell hemoglobin of the reticulocytes or erythrocytes are calculated from the measured cell-by-cell volume and hemoglobin concentration.
A reagent composition for treating mammalian red blood cells in an anticoagulated whole blood sample which can be subsequently effectively measured electrooptically, said reagent composition comprising a zwitterionic surfactant as the sole surfactant which is a sphering agent that eliminates orientational noise when said sample is mixed with said reagent composition and is subsequently subjected to flow cytometric measurement, said surfactant present at a concentration effective to substantially sphere the mature erythrocytes and the reticulocytes in said whole blood sample without inducing cell lysis after mixing with said sample for at least 2 minutes;
A reagent composition for the characterization of reticulocytes in an anticoagulated whole blood sample by flow cytometry;
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