Aldehyde dehydrogenase selectable markers for plant transformation
AssigneeCalgene, Inc.
InventorVirginia M. Ursin
Life of this patent
Estimating the term…
Abstract
The instant application provides a method of plant transformation in which plant cells are transformed with an aldehyde dehydrogenase gene capable of detoxifying a phytotoxic aldehyde selective agent. Transformed plant cells are cultured in the presence of the phytotoxic aldehyde selective agent to produce transformed shoots which may be regenerated to produce transformed plant cells containing the aldehyde dehydrogenase gene. The aldehyde dehydrogenase gene construct is linked to another gene construct of interest for expression in plant cells, wherein the aldehyde dehydrogenase gene acts as a selectable marker for transgenic plant cells containing the desired gene construct.
Claims21
A method of plant cell transformation, wherein said method comprises:
- introducing into cells of a plant species sensitive to growth inhibition by a phytotoxic aldehyde, a DNA construct comprising as operably linked components, a promoter functional in said plant cells, a DNA sequence encoding an aldehyde dehydrogenase capable of detoxifying said phytotoxic aldehyde, and a transcription termination region functional in said plant cells,
- culturing said plant cells in a plant growth medium comprising said phytotoxic aldehyde, and selecting transformed plant cells demonstrating resistance to said phytotoxic aldehyde.
A method of plant cell transformation, wherein said method comprises:
- introducing into cells of a plant species sensitive to growth inhibition by betaine aldehyde, a DNA construct comprising as operably linked components, a promoter functional in said plant cells, a DNA sequence encoding a betaine aldehyde dehydrogenase capable of detoxifying said betaine aldehyde, and a transcription termination region functional in said plant cells,
- culturing said plant cells in a plant growth medium comprising said betaine aldehyde, and selecting transformed plant cells demonstrating resistance to said betaine aldehyde.
A method of plant cell transformation, wherein said method comprises:
- introducing into cells of a plant species sensitive to growth inhibition by a phytotoxic aldehyde selected from the group consisting of acetaldehyde, formaldehyde, proprionaldehyde, butyraldehyde, and betaine aldehyde, a DNA construct comprising as operably linked components, a promoter functional in said plant cells, a DNA sequence encoding an aldehyde dehydrogenase capable of detoxifying said phytotoxic aldehyde and a transcription termination region functional in said plant cells,
- culturing said plant cells in a plant growth medium comprising said phytotoxic aldehyde, and selecting transformed plant cells demonstrating resistance to said phytotoxic aldehyde.
Dependencies are inferred from the claim text.