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US 5,633,151 AGranted
Enzymatic process for the isolation of erucic acid from vegetable oils
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorGerald P. McNeill
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Abstract
Erucic acid is produced by enzymatic hydrolysis, the reaction being catalyzed by lipase from Geotrichum candidum or Candida rugosa. Fatty acid substituents having carbon chains shorter than C22 are removed from triglycerides which also contain one or two erucic acid substituents, resulting in a glyceride fraction enriched in erucic acid and a free fatty acid fraction containing the hydrolyzed substituent.
Claims10
A method for the enzymatic production of a glyceride consisting essentially of erucic acid, said method comprising
- a) dissolving lipase in water or buffer,
- b) adding the dissolved lipase to an erucic acid-containing sample, with stirring, to form a reaction mixture,
- c) incubating the reaction mixture for a time sufficient for the hydrolysis of fatty acids other than erucic acid to occur, thereby forming a glyceride fraction consisting essentially of erucic acid and a free fatty acid fraction,
- d) separating the glyceride fraction from the free fatty acid fraction,
- wherein the glyceride is dierucin or monoerucin,
- wherein the lipase is Geotrichum candidum lipase or Candida rugosa lipase, and
- wherein the reaction is carried out at a temperature of from about 10.degree. C. to less than about 20.degree. C. when the lipase is Candida rugosa.
4 more elements
Dependencies are inferred from the claim text.