Toxoplasma gondii P28 gene and methods for its use
AssigneeResearch Institute of Palo Alto Medical Foundation
InventorsJeffrey B. Prince, Fausto G. De Araujo, Somesh D. Sharma, Jack S. Remington
Life of this patent
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Abstract
Genetic material encoding the P28 peptide of Toxoplasma gondii has been isolated and characterized. This genetic material allows the production of peptides for use in diagnosis or immunization or can itself be directly used in hybridization assays.
Claims16
An isolated DNA or RNA molecule, which comprises a nucleotide sequence coding for a P28 protein of Toxoplasma gondii or a peptide fragment of said P28 protein which is immunologically reactive with an antibody against Toxoplasma gondii or induces formation of an antibody or cell-mediated immune response against T. gondii.
An isolated polynucleotide, comprising at least 10 consecutive nucleotides selected from a first nucleotide sequence
- __________________________________________________________________________ T TTGCAATTCG CGTCGTTATC GCACGTTGTT TCTCTTCCCA CGAATAGTTG TTTTGATTAG ATATTGCTTC TTCTCCACAT ATCGCCTCAC AATGTTCGCC GTAAAACATT GTTTGCTGGT TGTTGCCGTT GGCGCCCTGG TCAACGTCTC GGTGAGGGCT GCCGAGTTTT CCGGAGTTGT TAACCAGGGA CCAGTCGACG TGCCTTTCAG CGGTAAACCT CTTGATGAGA GAGCAGTTGG A GGAAAAGGTG AACATACACC ACCACTCCCA GACGAGAGGC AACAAGAGCC AGAAGAACCG GTTTCCCAAC GTGCATCCAG AGTGGCAGAA CAACTGTTTC GCAAGTTCTT GAAGTTCGCT GAAAACGTCG GACATCACAG TGAGAAGGCC TTCAAAAAAG CAAAGGTGGT G, __________________________________________________________________________
- equivalent nucleotide sequences encoding the same amino acids as said first nucleotide sequence, complementary DNA nucleotide sequences, and complementary RNA nucleotide sequences wherein said DNA and RNA nucleotide sequences specifically hybridize to said first nucleotide sequence or equivalent sequence.
An isolated polynucleotide, comprising at least 10 consecutive nucleotides selected from a first nucleotide sequence
- __________________________________________________________________________ TCTCCACAT ATCGCCTCAC AATGTTCGCC GTAAAACATT GTTTGCTGGT TGTTGCCGTT GGCGCCCTGG TCAACGTCTC GGTGAGGGCT GCCGAGTTTT CCGGAGTTGT TAACCAGGGA CCAGTCGACG TGCCTTTCAG CGGTAAACCT CTTGATGAGA GAGCAGTTGG GTAAGTTGGC AAAAGTAATG ATAGAGGCAG GGGTTGAACG ATAGGCGGCT GCAGATTTGT ATAACACAAC ATGATGTAGC TGCCACGGTT TTTTTTCGGA GAGTGATGCC GTCTGACTGT TCATCGCACC CATGGGAGCT AGGGAGGTGC GCTTTCTGTG TGATATGTAT TGTCCTAGTC CAATTTCCCA CGCACTGTAG TGTCTTGAGA CTCGGTGCCA TGTAGAATTT TGTGTCTGCC TGCAGAGGAA AAGGTGAACA TACACCACCA CTCCCAGACG AGAGGCAACA AGAGCCAGAA GAACCGGTTT CCCAACGTGC ATCCAGAGTG GCAGAACAAC TGTTTCGCAA GTTCTTGAAG TTCGCTGAAA ACGTCGGACA TCACAGTGAG AAGGCCTTCA AAAAAGCAAA GGTGGTGGCA GAAAAAGGCT TCACCGCGGC AAAAACGCAC ACGGTTAGGG GTTTCAAGGT GGCCAAAGAA GCAGCTGGAA GGGGCATGGT GACCGTTGGC AAGAAACTCG CGAATGTGGA GAGTGACAGA AGCACTACGA CAACGCAGGC CCCCGACAGC CCTAATGGCC TGGCAGAAAC CGAGGTTCCA GTGGAGCCCC AACAGCGGGC CGCACACGTG CCCGTCCCAG ACTTTTCGCA GTAATGTTGA CTACGACGAA AGTGATGCGC AGGCTGGAAA GCCGCTGAAG G, __________________________________________________________________________
- equivalent nucleotide sequences encoding the same amino acids as said first nucleotide sequence, complementary DNA nucleotide sequences, and complementary RNA nucleotide sequences wherein said DNA and RNA nucleotide sequences specifically hybridize to said first nucleotide sequence or equivalent sequence.
Dependencies are inferred from the claim text.