Thermostable alkaline phosphatase of thermus thermophilus
AssigneeAmersham Life Science, Inc.
InventorsMaria Davis, Joseph Szasz
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Abstract
Alkaline phosphatase from Thermus thermophilus has been isolated. The enzyme has a pH optimum of greater than 10.5 and is stable to heating at 65.degree. C for 1 hour. The claimed invention relates to a method of detecting nucleic acids in a sample by providing a nucleic acid probe labelled with thermostable alkaline phosphatase from Thermus thermophilus; contacting the sample with said labelled nucleic acid; and detecting said nucleic acid in said sample by means of said thermostable alkaline phosphatase.
Claims1
Method for detecting nucleic acid in a sample comprising the step of providing a nucleic acid probe labelled with a thermostable alkaline phosphatase isolated from Thermus thermophilus;
- contacting the sample with said labelled nucleic acid;
- and detecting said nucleic acid in said sample by means of said thermostable alkaline phosphatase.
Dependencies are inferred from the claim text.