Method of producing a transgenic bovine or transgenic bovine embryo
AssigneePharming B.V.
InventorsHerman A. DeBoer, Rein Strijker, Herbert L. Heyneker, Gerard Platenburg, Sang H. Lee, Frank Pieper, Paul J. A. Krimpenfort
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Abstract
A method is disclosed for the production of a transgenic bovine or a transgenic bovine embryo comprising obtaining an ovum from bovine ovaries, maturing the ovum in vitro, fertilizing the mature ovum or ova in vitro to form a zygote, introducing a transgene into the zygote in vitro and maturing the zygote to a preimplantation stage embryo in vitro. To produce the transgenic bovine, the embryo is transplanted into a recipient female bovine, wherein the female bovine gestates the embryo to produce a transgenic bovine.
Claims29
A method of producing a transgenic bovine, the method comprising:
- obtaining an ovum from bovine ovaries;
- maturing the ovum in vitro;
- fertilizing the mature ovum in vitro to form a zygote;
- introducing a transgene into the zygote in vitro;
- maturing the zygote to a preimplantation stage embryo in vitro;
- and
- transplanting the embryo into a recipient female bovine, wherein the female bovine gestates the embryo to produce a transgenic bovine.
A method of producing a transgenic bovine embryo, the method comprising:
- obtaining an ovum from bovine ovaries;
- maturing the ovum in vitro;
- fertilizing the mature ovum in vitro to form a zygote;
- and
- introducing a transgene into the zygote, wherein the transgene integrates into the genome of the zygote to form the transgenic bovine embryo.
Dependencies are inferred from the claim text.