Process for the expression of herpes simplex virus type 1 glycoprotein E and method of use
AssigneeCedars-Sinai Medical Center
InventorsAnthony B. Nesburn, Steven L. Wechsler, Homayon Ghiasi
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Abstract
A process for obtaining Herpes Simplex virus type 1 (HSV-1) glycoprotein E (gE) from cells which have been infected or transformed with a recombinant Baculovirus is disclosed. The gE produced is then isolated and purified for use in immunotherapy against HSV infections.
Claims21
A vaccine against Herpes Simplex Virus-1 infection, comprising Herpes Simplex Virus Type 1 (HSV-1) gE produced by the expression of a recombinant baculovirus having a genetic construct which comprises:
- a) a gene sequence encoding at least one of a full length HSV-1 gE (SEQ ID No. 1) and HSV-1 gE truncated by removal of a fragment encoding the transmembrane anchor sequence;
- and
- b) a promoter sequence wherein said gE gene sequence is functionally linked to the regulatory elements of said promoter, and wherein said promoter is Operative in a baculovirus expression system.
A process for preparing a vaccine against Herpes Simplex Virus-1 infection, comprising the steps of:
- preparing a recombinant baculovirus having a genetic construct which comprises a gene sequence encoding at least one of a full length Herpes Simplex Virus Type 1 (HSV-1) gE (SEQ ID No. 1) and HSV-1 gE truncated by removal of a fragment encoding the transmembrane anchor sequence and a promoter sequence wherein said gE gene sequence is functionally linked to the regulatory elements of said promoter and wherein said promoter is operative in a baculovirus expression system:
- introducing the recombinant baculovirus into a first set of host cells;
- culturing the host cell;
- and
- recovering said HSV-1 gE from the culture.
A vaccine for the treatment or prevention of Herpes Simplex Virus-1 infection comprising at least one of full length Herpes Simplex Virus Type 1 (HSV-1) gE and HSV-1 gE truncated by removal Of the transmembrane anchor sequence.
Dependencies are inferred from the claim text.