Fluoresence detection using texaphyrins

A method of determining the localization site of a water-soluble detectable texaphyrin, the method comprising the step of observing fluorescence of the texaphyrin wherein fluorescence indicates the presence of the texaphyrin, the detectable texaphyrin having structure A:

• ##STR6## wherein, M is H, a divalent metal cation or a trivalent metal cation;
• R.sub.1 -R.sub.4 and R.sub.6 -R.sub.9 are independently hydrogen, halide other than iodide, hydroxyl, alkyl, aryl, haloalkyl other than iodoalkyl, nitro, formyl, acyl, hydroxyalkyl, oxyalkyl, oxyhydroxyalkyl, saccharide, aminoalkyl, oxyaminoalkyl, carboxy, carboxyalkyl, carboxyamidealkyl, a site-directing molecule or a couple to a site-directing molecule;
• R.sub.5 and R.sub.10 are independently hydrogen, alkyl, aryl, hydroxyalkyl, oxyalkyl, oxyhydroxyalkyl, aminoalkyl, oxyaminoalkyl, carboxyalkyl, carboxyamidealkyl or a couple to a site-directing molecule;
• where R.sub.6 and R.sub.9 are other than hydrogen, then R.sub.5 and R.sub.10 are hydrogen or methyl;
• where R.sub.5 and R.sub.10 are other than hydrogen, then R.sub.6 and R.sub.9 are hydrogen, hydroxyl, or halide other than iodide;
• and
• N is 0, 1 or 2.

A method of determining the localization site of a water-soluble detectable texaphyrin, the method comprising the step of observing fluorescence of the texaphyrin wherein fluorescence indicates the presence of the texaphyrin, the detectable texaphyrin having structure B:

• ##STR7## wherein, R.sub.1 -R.sub.4 and R.sub.6 -R.sub.9 are independently hydrogen, halide other than iodide, hydroxyl alkyl, aryl, haloalkyl other than iodoalkyl, nitro, formyl, acyl, hydroxyalkyl, oxyalkyl, oxyhydroxyalkyl, saccharide, aminoalkyl, oxyaminoalkyl, carboxy, carboxyalkyl, carboxyamidealkyl, a site-directing molecule or a couple to a site-directing molecule;
• R.sub.5 and R.sub.10 are independently hydrogen, alkyl, aryl, hydroxyalkyl, oxyalkyl, oxyhydroxyalkyl, aminoalkyl, oxyaminoalkyl, carboxyalkyl, carboxyamidealkyl or a couple to a site-directing molecule;
• R.sub.11 is alkyl, alkenyl, oxyalkyl or hydroxyalkyl having up to about 3 carbon atoms and having rotational flexibility around a first-bound carbon atom;
• where R.sub.6 and R.sub.9 are other than hydrogen, then R.sub.5 and R.sub.10 are hydrogen or methyl;
• and
• where R.sub.5 and R.sub.10 are other than hydrogen, then R.sub.6 and R.sub.9 are hydrogen, hydroxyl, or halide other than iodide.