Back to results
US 5,631,348 AGranted
Protein sequence of the plant toxin gelonin
AssigneeResearch Development Foundation
InventorsMichael Rosenblum, William J. Kohr, Bharat Aggarwal
Life of this patent
Estimating the term…
Abstract
This invention relates to substantially purified gelonin, toxic fragments thereof, the DNA sequences encoding gelonin and use of the DNA for producing, by recombinant technology, gelonin, toxic fragments thereof and fusion proteins. More specifically, the invention relates to the primary amino acid sequence of gelonin, and of the DNA encoding said gelonin and the production of synthetic gelonin and toxic fragments thereof.
Claims2
Substantially pure gelonin toxin having the amino acid sequence:
- ##STR1## or a fragment or derivative thereof, said fragment or derivatives or having an activity which inhibits cellular protein synthesis but does not bind to a cell surface receptor.
A DNA sequence of the formula:
- __________________________________________________________________________ GGNYTNGAYA CNGTNWSNTT YWSNACNAAR GGNGCNACNT AYATHACNTA YGTNAAYTTY 60 YTNAAYGARY TNMGNGTNAA RYTNAARCCN GARGGNAAYW SNCAYGGNAT HCCNYTNYTN 120 MGNAARGGNG AYGAYCCNGG NAARTGYTTY GTNYTNGTNG CNYTNWSNAA YGAYAAYGGN 180 CARYTNGCNG ARATHGCNAT HGAYGTNACN WSNGTNTAYG TNGTNGGNTA YCARGTNMGN 240 AAYMGNWSNT AYTTYTTYAA RGAYGCNCCN GAYGCNGCNT AYGARGGNYT NTTYAARAAY 300 ACNATHAARA AYCCNYTNYT NTTYGGNGGN AARACNMGNY TNCAYTTYGG NGGNWSNTAY 360 CCNWSNYTNG ARGGNGARAA RGCNTAYMGN GARACNACNG AYYTNGGNAT HGARCCNYTN 420 MGNATHGGNA THAARAARYT NGAYGARAAY GCNATHGAYA AYTAYAARCC NACNGARATH 480 GCNWSNWSNY TNYTNGTNGT NATHCARATG GTNWSNGARG CNGCNMGNTT YACNTTYATH 540 GARAAYCARA THMGNAAYAA YTTYCARCAR MGNATHMGNC CNGCNAAYAA YACNATHWSN 600 YTNGARAAYA ARTGGGGNAA RYTNWSNTTY CARATHMGNA CNWSNGGNGC NAAYGGNATG 660 TTYWSNGARG CNGTNGARYT NGARMGNGCN AAYGGNAARA ARTAYTAYGT NACNGCNGTN 720 GAYCARGTNA ARCCNAARAT HGCNYTNYTN AARTTYGTNG AYAARGAYCC NGAR 774 wherein R = A or G K = G or T N = any Y = C or T M = A or C S = C or G B = C, G, or T V = A, C, or G W = A or T D = A, G, or T H = A, C, or T X = unknown __________________________________________________________________________
- or a fragment or derivative thereof, said fragment or derivative coding for gelonin or for a polypeptide having an activity which inhibits cellular protein synthesis but does not bind to a cell surface receptor.
Dependencies are inferred from the claim text.