Thermoreversible hydrogels comprising linear copolymers and their use in electrophoresis
AssigneeSoane BioScience
InventorsAlexander P. Sassi, Shi Lin, M. Goretty Alonso-Amigo, Herbert H. Hooper
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Abstract
Thermoreversible hydrogels comprsing non-ionic, linear copolymers, and methods of their use in electrophoresis, are provided. The subject copolymers comprise polyacrylamide backbones, where a portion of the acrylamide monomeric units compose hydrogen bonding groups as N-substituents. Combination of the subject copolymers with an aqueous phase provides thermoreversible hydrodgels which find use as separation media in electrophoretic applications.
Claims18
A non-ionic, linear copolymer capable of forming a thermoreversible hydrogel when combined with an aqueous phase, said copolymer comprising:
- a polyacrylamide backbone, wherein 55 to 95 weight percent ratio of the acrylamide monomeric units of said copolymer comprise N-substituent groups capable of hydrogen bonding.
A non-ionic, linear copolymer capable of forming a thermoreversible hydrogel when combined with an aqueous phase, said copolymer comprising:
- first and second monomeric units in a weight percent ratio of from about 55:45 to about 95:5, wherein said first monomeric unit is of the formula:
- ##STR4## wherein:
- X is H or CH.sub.3,
- Y is a bond or a linking group of from 1 to 6 carbon atoms;
- and
- Z is a group comprising a hydrogen bonding moiety, wherein said hydrogen bonding moiety is of the formula:
- ##STR5## wherein:
- A is C or a heteroatom;
- D is O or S;
- and
- R.sub.1 is H or an aliphatic substituent of up to 10 carbon atoms;
- and
- said second monomeric unit is acrylamide.
A copolymer of acrylylglycinamide and acrylamide, wherein the weight percent ratio of acrylylglycinamide to acrylamide of said copolymer ranges from about 55:45 to about 95:5.
A thermoreversible hydrogel suitable for use as a separation medium for electrophoresis, said hydrogel comprising:
- a non-ionic, linear copolymer comprising a polyacrylamide backbone, wherein 55 to 95 weight percent ratio of the acrylamide monomeric units of said copolymer comprise N-substituent groups capable of hydrogen bonding;
- and
- a continuous fluid phase.
A thermoreversible hydrogel suitable for use as a separation medium for electrophoresis, said hydrogel comprising:
- a non-ionic, linear copolymer comprising first and second monomeric units in a weight percent ratio of from about 55:45 to about 95:5, wherein said first monomeric unit is wherein said first monomeric unit is of the formula:
- ##STR6## wherein:
- X is H or CH.sub.3,
- Y is a bond or a linking group of from 1 to 6 carbon atoms;
- and
- Z is a group comprising a hydrogen bonding moiety, wherein said hydrogen bonding moiety is of the formula:
- ##STR7## wherein:
- A is C or a heteroatom;
- D is O or S;
- and
- R.sub.1 is H or an aliphatic substituent of up to 10 carbon atoms;
- and
- said second monomeric unit is acrylamide;
- and
- a continuous liquid phase.
A thermoreversible hydrogel suitable for use as a separation medium for electrophoresis, said hydrogel comprising:
- a polyacrylamide backbone, wherein a portion of the acrylamide monomeric units of said copolymer comprise N-substituent groups capable of hydrogen bonding;
- an additional, non-proteinaceous linear polymer;
- and
- an aqueous phase.
Dependencies are inferred from the claim text.