Method for producing in vivo delivery of therapeutic agents via liposomes
OwnerVictor J. Dzau, Yasufumi Kanedainventors
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Abstract
Methods and compositions are provided for intracellular transfer of a wide variety of agents, by using Sendai virus comprising liposomes having various compositions in the liposome lumen. A preferred method for preparing the liposomes provides for enhanced levels of luminal concentrations, as well as incorporation of high molecular weight molecules. The method comprises fusing liposomes, where one liposome comprises the Sendai virus proteins and the other liposome comprises the luminal composition. The subject methods find particular application with intranuclear transfer of nucleic acids, more particularly with cells of the vasculature.
Claims10
A method for producing liposomes for fusion with cells, said method comprising:
- agitating in a first aqueous medium purified HN and F proteins of Sendai virus with liposome forming lipids to form first liposomes;
- agitating in a second aqueous medium an agent of interest with liposome forming lipids to form second liposomes,
- wherein at least 25 mole % of the total lipids is cationic lipids;
- combining said first and second liposomes under fusing conditions to provide liposomes for fusion with cells.
A method for producing liposomes for fusion with cells, said method comprising:
- agitating in a first aqueous medium purified HN and F with liposome forming lipids to form first liposomes;
- agitating in a second aqueous medium nucleic acids and a high mobility group protein with liposome forming lipids to form second liposomes,
- wherein at least 25 mole % of the total lipids is cationic lipids;
- combining said first and second liposomes under fusing conditions to provide liposomes for fusion with cells.
Dependencies are inferred from the claim text.