Pollenosis-inducing polypeptide, process for preparing the same, and uses thereof
AssigneeKabushiki Kaisha Hayashibara Sibutsu Kagaku Kenkyujo
InventorsKeizo Kohno, Manami Sawatani, Masashi Kurimoto
Life of this patent
Estimating the term…
Abstract
A polypeptide having a molecular weight of 40,000.+-.5,000 daltons and an isoelectric point of 9.5.+-.0.5 is prepared from a cedar pollen. The polypeptide which induces pollenosis can be suitably used as desensitization agent because it induces immunoglobulin antibody which is effective for desensitization, but does not substantially induce immunoglobulin E antibody, a major factor causative of side effects including anaphylaxis shock. Therefore, the polypeptide can be advantageously used in the treatment, prevention and/or diagnosis of pollenosis.
Claims17
A purified polypeptide having the following physicochemical properties:
- (1) Molecular weight
- 40,000.+-.5,000 daltons on sodium dodecyl polyacrylamide gel electrophoresis (SDS-PAGE);
- (2) Isoelectric point (pI)
- 9.5.+-.0.5 on isoelectrophoresis;
- (3) Partial amino acid sequence containing the N-terminal as an N-terminal sequence Ser-Arg-His-Asp-Ala-Ile-, or Arg-Lys-Val-Glu-His-Ser-Arg-His-Asp-Ala-Ile- (SEQ ID NO:
- 1);
- (4) Ultraviolet absorption spectrum
- Exhibiting the maximum absorption spectrum at a wave length of around 280 nm;
- (5) Solubility:
- Soluble in water, physiological saline and phosphate buffer;
- (6) Biological activity
- Inducing pollenosis by binding to immunoglobulin E antibody collected from blood of a patient with pollenosis;
- and
- (7) Stability
- Being inactivated in an aqueous solution (pH 7.2) when incubated at 100.degree. C. for 10 minutes;
- Substantially not losing its activity in an aqueous solution (pH 7.2) when allowed to stand at 4.degree. C. for one month.
Dependencies are inferred from the claim text.