Detergent compositions comprising a modified subtilisin
AssigneesNovo Nordisk A/S, Unilever PLC
InventorsSven Branner, Sven Hastrup, Nina Eriksen, Poul Lindegaard, Ole H. Olsen, Eric Casteleijn, Maarten R. Egmond, Johan Haverkamp, Wouter Musters, Jakob de Vlieg
Life of this patent
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Abstract
This invention relates to enzymes, to rDNA techniques applicable for example to their production, to mutated genes, vectors and mutant and transformed microorganisms useful in their production, and to their uses including for example enzymatic detergent and cleaning compositions containing them.
Claims48
A modified subtilisin comprising a mutation in an amino acid sequence of a subtilisin, wherein the mutation is a substitution of a tyrosine at position 167, numbered according to the amino acid sequence of the mature subtilisin BPN', with a threonine or tryptophan.
A detergent composition comprising a surfactant and a modified subtilisin, wherein the modified subtilisin comprises a mutation in an amino acid sequence of a subtilisin at a position numbered according to the amino acid sequence of the mature subtilisin BPN', wherein the mutation is selected from the group consisting of:
- (a) a histidine at position 17 substituted by a glutamine,
- (b) a histidine at position 39 substituted by a serine,
- (c) a glutamic acid at position 54 substituted with an aspartic acid,
- (d) a histidine at position 120 substituted with an asparagine,
- (e) a tyrosine at position 167 substituted with a glutarnic acid,
- (f) a tyrosine at position 167 substituted with a phenylalanine,
- (g) a tyrosine at position 171 substituted with a phenylalanine,
- (h) a tyrosine at position 171 substituted with a valine,
- (i) a tyrosine at position 192 substituted with a glutamic acid,
- (j) a tyrosine at position 192 substituted with a phenylalanine,
- (k) a tyrosine at position 209 substituted with a phenylalanine,
- (l) a tyrosine at position 214 substituted with a phenylalanine,
- (m) a histidine at position 226 substituted with a serine, and
- (n) a tyrosine at position 263 substituted with a phenylalanine.
Dependencies are inferred from the claim text.