Lipid-modified serum free media
AssigneeCreative BioMolecules, Inc.
InventorsPaul G. Marshall, Patrick M. Guertin
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Abstract
Methods are provided for producing serum-free, animal cell culture media which have enhanced cell culture properties, such as cell growth, cell viability and protein production properties. The cell culture properties of standard serum-free media formulations, which include compositions such as inorganic salts, glucose, fatty acids, amino acids and vitamins, can be enhanced according to the methods of the invention, by providing the media with an additive. The additive in one embodiment can be produced by the acid or base catalyzed hydrolysis of a phosphoglyceride.
Claims27
An improved serum-free cell culture medium having improved cell viability or recombinant protein production properties, wherein the improvement comprises an additive formed by alchohol hydrolysis of an intact phosphoglyceride ester defining a substituted glycerol wherein the hydroxyl group at each of C1 and C2 is esterified to the carboxyl group of a fatty acid and the C3 is esterified to a phosphate, the alcohol hydrolysis being sufficient to release one alkylated fatty acid ester from said phosphoglyceride.
A method of improving the cell cultivation or biologicals production properties of a serum-free cell culture medium comprising the step of:
- adding to a serum-free cell culture medium an additive formed by alcohol hydolysis of an intact phosphoglyceride ester defining a substituted glycerol wherein the hydroxyl group at each of C1 and C2 is esterified to the carboxyl group of a fatty acid and the C3 is esterified to a phosphate, the alcohol hydrolysis being sufficient to release one alkylated fatty acid ester from said phosphoglyceride.
Dependencies are inferred from the claim text.