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US 5,631,152 AGranted
Rapid and efficient regeneration of transgenic plants
AssigneeMonsanto Company
InventorsJoyce E. Fry, Hua-ping Zhou
Life of this patent
Estimating the term…
Abstract
A rapid transformation regeneration system is disclosed. This system takes two-three months to obtain transgenic plants. Transformation efficiencies are very high. This system also has been demonstrated with several different selecting systems and is particularly useful for transforming wheat.
Claims12
A method for regenerating a fertile transformed wheat plant to contain foreign DNA comprising the steps of:
- (a) producing regenerable tissue from said plant to be transformed:
- (b) transforming said regenerable tissue with said foreign DNA where said foreign DNA comprises a selectable DNA sequence, where said sequence functions in a regenerable tissue as a selection device;
- (c) between about one day to about 21 days after step b) placing said regenerable tissue from step b) in a medium capable of producing shoots from said tissue wherein said medium further contains a compound used to select regenerable tissue containing said selectable DNA sequence to allow identification or selection of the transformed regenerated tissue;
- (e) after at least one shoot has formed from the selected tissue of step c), transferring said shoot to a second medium capable of producing roots from said shoot to produce a plantlet;
- and,
- (e) growing said plantlet into a fertile, transgenic wheat plant wherein the foreign DNA is transmitted to progeny plants in mendelian fashion.
3 more elements
A method for regenerating a fertile transformed wheat plant to contain foreign DNA comprising the steps of:
- (a) producing embryogenic calli from said plant to be transformed;
- (b) transforming said calli with said foreign DNA where said foreign DNA comprises a selectable DNA sequence, where said sequence functions in a regenerable tissue as a selection device;
- (c) between about one day to about 21 days after step b) placing said regenerable tissue from step b) in a medium capable of producing shoots from said tissue where said medium further contains a compound used to select the transformed regenerable tissue containing said selectable DNA sequences to allow identification or selection of the transformed regenerated tissue;
- (d) after at least one shoot has formed from the selected tissue of step c), transferring said shoot to a second medium capable of producing roots from said shoot to produce a plantlet;
- and,
- (e) growing said plantlet into a fertile transgenic wheat plant wherein the foreign DNA is transmitted to progeny plants in mendelian fashion.
3 more elements
Dependencies are inferred from the claim text.