US 5,631,144 AGranted

Application of novel DNA fragments as a coding sequence for a signal peptide for the secretion of mature proteins by recombinant yeast, expression cassettes, transformed yeast and corresponding process for the preparation of proteins

AssigneeTransgene S.A.

InventorsYves Lemoine, Martine Nguyen, Tilman Achstetter, Jean-Marc Reichhart

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Abstract

The invention relates to new DNA fragments and to their application as a DNA coding fragment for a signal peptide which can be used for the secretion of proteins, said peptide including a sequence of amino-acids which show a degree of correspondence of at least 60% with the sequence of amino-acids (I) or (II), preferably with the sequence (II). The sequences (I) and (II) are as follows: (I) Arg-Phe-Ser-Thr-Thr-Leu-Ala-Thr-Ala-Ala-Thr-Ala-Leu-Phe-Phe-Thr-Ala-Ser-Gl n. (II) Arg-Phe-Ser-Thr-Thr-Leu-Ala-Thr-Ala-Ala-Thr-Ala-Leu-Phe-Phe-Thr-Ala-Ser-Gl n-Val-Ser-Ala.

Claims25

Dependent claims

An isolated DNA fragment comprising a DNA sequence which codes for a peptide comprising the amino acid sequence (III) (SEQ ID NO:5) R.sub.1 -R.sub.2 -R.sub.3 -Thr-Thr-R.sub.4 -Ala-Thr-Ala-Ala-Thr-Ala-Leu-Phe-Phe-Thr-Ala-R.sub.5 -R.sub.6 in which

R.sub.1 is an amino acid selected from Arg and Lys
R.sub.2 and R.sub.6 are each an amino acid selected independently from Ala, Asn, Cys, Gln, Gly, His, Ile, Leu, Met, Phe, Pro, Set, Thr, Trp, Tyr, and Val,
R.sub.3 and R.sub.5 are each an amino acid selected independently from Asp, Gly, Asn, Pro, and Ser, and
R.sub.4 is an amino acid selected from Val, Leu, Ala, Cys, Phe, Ile and Met, wherein the peptide acts as a signal sequence.

1 more element

Dependencies are inferred from the claim text.

Facts

Application: 08/385,375Filed 7 Feb 1995 · continuation
Priority: FR 89 0568728 Apr 1989
Granted: 20 May 1997
Term ends: Not estimatedThe source records don’t support an estimate.

Classification14 codes

C07K 14/43563
From invertebrates: from insects
C07K 14/815
Protease inhibitors: from leeches, e.g. hirudin, eglin
C12N 9/2402
Acting on glycosyl compounds: hydrolysing O- and S- glycosyl compounds
C12N 15/625
DNA sequences coding for fusion proteins: containing a sequence coding for a signal sequence